斑点免疫金渗滤法检测边虫病的研究  

STUDY ON DETECTION OF ANAPLASMOSIS BY DOT IMMUNOGOLD FILTRATION ASSAY

作　　者：徐梅倩[1] 陈克喜[1] 张北民[1] 吴鉴三[1] 张小正[1] 

机构地区：[1]中国农科院上海家畜寄生虫病研究所,农业部动物检疫所

出　　处：《中国兽医寄生虫病》1996年第3期10-12,共3页Chinese Journal of Veterinary Parasitology

摘　　要：应用自行制备的直径２５ｎｍ～３０ｎｍ的胶体金标记ＳＰＡ，建立了检测抗边虫抗体的斑点免疫金渗滤试验（ＤＩＧＦＡ），同时作与补体结合试验（ＣＦＴ）、团集凝集试验（ＣＡＴ）比较试验，结果表明ＤＩＧＦＡ是一种检测边虫病抗体的敏感性高、特异性强、重复性好的快速、简便易行方法。SPA labelled with 25-30 nm diametral colloidal gold was prepared in this laboratory. Dot immunogold filtration assay (DIGFA) was established for detection anti-Anaplasma marginale antibody and were comparied with complement-fixation test (CFT), conglutinin agglutination test (CAT) under the same conditions. The results showed that DIGFA is a highly sensitive, specific.reproduceable.facile and rapid method for the detection of anti-Anaplasma marginale antibody.

关 键 词：边虫 胶体金探针 斑点免疫 金渗滤试验 

分 类 号：S855.9[农业科学—临床兽医学]