急性毒性体外筛选方法的比较  

Comparison the screening methods of the acute toxicity in vitro

作　　者：王征[1] 张天宝[1] 朱玉平[1] 谢宗华[1] 

机构地区：[1]第二军医大学卫生毒理学教研室,上海200433

出　　处：《卫生研究》2006年第3期286-288,共3页Journal of Hygiene Research

基　　金：国家973课题分题(No.2002CB512901)

摘　　要：目的比较4种急性毒性的体外筛选方法,为建立高通量的急性毒性筛选方法提供依据。方法选用人HepG2细胞,运用96孔板体外细胞培养技术,对47种已知体内急性毒性的化合物用4种细胞毒性的检测方法及5个评价指标进行检测,并将体外评价指标与体内评价指标LD50进行分析比较。结果MTT、中性红、LDH及刃天青4种检测方法中,以中性红法检测结果与体内LD50的相关性最好,评价指标中4种检测方法均以IC45与LD50的相关性最高。结论选用人HepG2细胞,运用微孔板体外细胞培养技术,4种细胞毒性检测方法中以中性红法方法最好,评价指标以IC45为宜,可作为建立高通量的急性毒性筛选方法的依据。Objective To provide the proof for establishing the high throughput screening method of the acute toxicity by comparing the 4 screening methods of the acute toxicity in vitro. Methods HepG2 cells, plated in 96-well plates, were treated with 47 compounds and we detected the cytotoxicity using 4 methods and calculated the IC50, IC45, IC40, IC35, IC30, and these values were compared with the LD50. Results The IC45 from the neutral red method has the best relativity with the LD50 . Conclusion Plating HepG2 cells in 96-well plates, the cytotoxicity be detected using the neutral red method and the IC45 was calculated. It is considered to provide the proof for establishing the high throughput screening method of the acute toxicity in vitro.

关 键 词：急性毒性 体外 筛选 

分 类 号：R114[医药卫生—卫生毒理学]