哈伯因对水胺硫磷和辛硫磷染毒小鼠的神经病理改变的影响  

Effect of HuperzineA on neurophathologic change of mouse exposed by isocarbophos and phoxim

作　　者：谢广云[1] 刘黎[1] 王建[1] 赵文锦[1] 孙金秀[1] 

机构地区：[1]中国疾病预防控制中心职业卫生与中毒控制所,北京100050

出　　处：《卫生研究》2006年第3期339-341,共3页Journal of Hygiene Research

基　　金：国家科技攻关项目(No.2001BA704B06)

摘　　要：目的观察哈伯因(HupA)对水胺硫磷和辛硫磷急性染毒小鼠中枢神经系统的影响。方法染毒组和预防处理组均经口给予一定剂量水胺硫磷或辛硫磷,预防处理组染毒前2h经口给予HupA,对照组经口给予吐温-80或花生油。染毒24h后采用原位整体灌注法固定动物,取脑,做冠状切片,进行HE染色和甲苯胺蓝染色,用Image Pro-Plus720进行神经病理组织学图像分析。结果水胺硫磷和辛硫磷染毒组动物均见到大脑皮质水肿、神经锥体细胞急性水肿、海马锥体细胞急性水肿,尼氏体减少。预防处理组部分动物虽见到大脑皮质水肿、神经锥体细胞急性水肿,而病变程度比染毒组明显减轻。结论HupA对水胺硫磷和辛硫磷染毒小鼠神经病理改变有一定的保护作用。Objective In order to assessment the Huperzine A（HupA） on CNS of mice exposed by phoxim and isocarbophos. Methods One group was given tween-80 or peanut oil as control, One group was given phoxim or isocarbophos alone as phoxim or isocarbophos group. The other group was given HupA 2h before phoxim or isocarbophos exposure as HupA protective group. Twenty-four hours after exposure, the whole body perfusion in situ technique was performed for tissue fixation. The whole brain was removed and adjacent coronal sections was obtained. The sections of brain were stained with hematoxylin and eosin （HE） and toluidine blue. Under optic microscope images were collected and analysed. Results The extensive lesions including cortex oedema, pyramidal cell oedema, and nissal body numbers reduced were observed in phoxim or isocarbophos group. The animals of HupA protective group were observed cortex oedema, pyramidal cell oedema, but the lesional degree was lighter obviously than phoxim or isocarbophos group. Conclusion It was suggested that the HupA could have the protective effect of neuropathologic changes exposed by phoxim and isocarbophos.

关 键 词：哈伯因 水胺硫磷 辛硫磷 神经病理改变 

分 类 号：R949.6[医药卫生—药剂学] R595.4[医药卫生—药学]