人骨关节炎滑膜成纤维细胞差异蛋白质谱初步分析  

Analyses of differentially expressed proteins of human synovial fibroblasts obtained from osteoarthritis

作　　者：柏干苹[1] 周丽娜[1] 贺伟峰[1] 罗高兴[1] 陈烯伟[1] 易绍萱[1] 胡晓红[1] 石东文[1] 陈光兴[2] 方勇飞[3] 吴军[1] 

机构地区：[1]第三军医大学西南医院全军烧伤研究所 [2]第三军医大学西南医院关节外科中心,重庆400038 [3]第三军医大学西南医院风湿科,重庆400038

出　　处：《第三军医大学学报》2006年第11期1221-1223,共3页Journal of Third Military Medical University

基　　金：疾病蛋白质组学重庆市市级重点实验室专项基金资助项目(2004)~~

摘　　要：目的应用双向凝胶电泳-质谱法分析正常人及骨关节炎(osteoarthritis,OA)患者滑膜成纤维细胞蛋白质组表达差异,探讨差异蛋白在骨关节炎发病中的作用。方法采用固相pH梯度(immob ilized pH grad ient,IPG)双向凝胶电泳(two-d imensional gel electrophoresis,2-DE)分离正常人及骨关节炎患者滑膜成纤维细胞总蛋白质,银染显色,PDQuest2-DE软件分析图像,比较2种细胞蛋白质组表达差异,对差异蛋白质点用基质辅助激光解析电离飞行时间质谱进行鉴定。结果胶图差异分析显示36个表达水平存在明显差异的蛋白质,选取15个点进行质谱鉴定,鉴定出6个表达上调蛋白质。结论正常人及骨关节炎患者滑膜成纤维细胞表达蛋白质组存在差异。这些差异蛋白可能参与骨关节炎的发病。Objective To understand the possible roles of some proteins expressed by human synovial fibroblasts in the pathogenesis of osteoarthritis by means of 2-DE and bioinformatics. Methods The difference of synovial fibroblasts protein expression between osteoarthritis patients and healthy controls was analyzed by 2- DE. The differential expression spots were identified by matrix-assisted laser desorption ionization-time-of-flight mass spectrometry （MALDI-TOF-MS）, followed by bioinformatics analysis. Results Gel-image analysis revealed that there were 36 differential protein spots. A total of 15 differential protein spots were identified by MALDI-TOF-MS, of which 6 spots were much more intensive in osteoarthritis patients than normal ones. Conclusion These differential proteins based on bioinformatics analysis might be involved in synovitis in osteoarthritis patients.

关 键 词：骨关节炎 滑膜成纤维细胞 蛋白质组 双向电泳 

分 类 号：R329.24[医药卫生—人体解剖和组织胚胎学] R341[医药卫生—基础医学]