β_2-BKR基因和AGT基因多态性与原发性高血压的相关性研究  

Association of genetic polymorphism of β_2-bradykinin receptor gene and AGT gene with essential hypertension in Shenzhen population

作　　者：董会奕[1] 李秋荣[1] 王勤[1] 罗振国[2] 

机构地区：[1]北京大学深圳医院心血管内科,深圳518036 [2]深圳大学生命科学学院,深圳518060

出　　处：《第三军医大学学报》2006年第11期1252-1254,共3页Journal of Third Military Medical University

摘　　要：目的调查缓激肽β2受体(β2-bradyk in in receptor,β2-BKR)基因和血管紧张素原(angiotensinogen,AGT)基因多态性与原发性高血压(essential hypertension,EH)的关系。方法采用病例-对照研究。用MS-PCR和PCR-SSCP方法检测深圳地区EH 97例(EH组)和血压正常者87例(NT组)的β2-BKR基因-58T/C多态性和AGT基因M235T多态性。结果EH组β2-BKR CC基因型(0.36)和C等位基因频率(0.60)显著高于NT组(0.14,P=0.000;0.43,P=0.001)。EH组CC+MT(0.19)、TC+MM(0.18)基因型显著高于NT组(0.06,P=0.009;0.05,P=0.006)。β2-BKR CC基因型者EH的相对危险度增加(OR=1.913,95%CI:1.913-3.049,P=0.006)。EH组AGT基因型分布与NT组相比无显著性差异(P=0.091),但M等位基因频率(0.55)显著高于NT组(0.44,P=0.037)。结论β2-BKR-58T/C多态性与深圳地区人群的EH相关,CC基因型可能与EH危险增加有关,C等位基因可能与AGTM235T多态性存在基因-基因间的协同作用。Objective To investigate the association between β2-bradykinin receptor （β2-BKR） gene and angiotensinogen gene polymorphism and essential hypertension. Methods The case-control study was conducted using MS-PCR and PCR-SSCP techniques to explore the β2-BKR gene-58T/C polymorphism and the AGT gene M235T polymorphism in 97 essential hypertensives and 87 normotensives of Shenzhen. Results The genotypes distribution of CC and the alletic frequency of C in EH cases were significantly higher than in controls （0.36 vs 0. 14, P =0.000; 0.60 vs 0.43, P =0.001）. The genotype distributions of CC ＋MT and TC ＋MM in EH cases were significantly higher than controls （0.19 vs 0. 06, P =0.009; 0.18 vs 0. 05, P =0. 006）. The odds ratio for those exposed to CC genotype was 1. 913, 95% confidence interval was 1. 913 to 3. 049, P = 0.006. No significant difference was seen in the genotype distribution of angiotensinogen gene between EH cases and controls （P =0. 091 ）, but the alletic frequency of M in EH cases was significantly higher than controls （0.55 vs 0.44, P = 0. 037 ）. Conclusion The 132-BKR gene-58T/C polymorphism might be associated with essential hypertension in Shenzhen population. CC genotype might be associated with the increased risk of EH and C allele might have a gene-gene synergetic effect with M235T polymorphism.

关 键 词：原发性高血压 缓激肽β2受体基因 血管紧张素原基因 基因多态性 

分 类 号：R181.33[医药卫生—流行病学] R394[医药卫生—公共卫生与预防医学]