罗哌卡因和布比卡因对大鼠海马神经元谷氨酸电流的影响  

Effects of Ropivacaine and Bupivacaine on glutamate-evoked currents in cultured rat hippocampal neurons

作　　者：姜雨鸽[1] 米卫东[1] 徐龙河[1] 张宏[1] 

机构地区：[1]中国人民解放军总医院麻醉科,北京100853

出　　处：《中国临床药理学与治疗学》2006年第4期410-413,共4页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：国家自然科学基金项目(№30300327)

摘　　要：目的:观察和比较不同浓度的局麻药罗哌卡因和布比卡因对大鼠海马神经元谷氨酸诱发电流的作用,以探讨罗哌卡因神经毒性低的可能机制。方法:分离大鼠海马神经元并培养,利用全细胞膜片钳技术记录细胞谷氨酸诱发电流,并观察罗哌卡因和布比卡因对谷氨酸电流的影响。结果:谷氨酸(100μmol.L-1)可在海马神经元细胞上诱发出一内向电流,并且此电流可被非N-甲基-天门冬氨酸(NMDA)受体拮抗剂DNQX所阻断;布比卡因(10、50、100μmol.L-1)和罗哌卡因(10、50、100μmol.L-1)均可使海马神经元细胞的谷氨酸诱发电流明显降低;罗哌卡因高浓度(501、00μmol.L-1)时对谷氨酸诱发电流的降低幅度显著高于布比卡因高浓度时。结论:布比卡因和罗哌卡因对大鼠海马神经元细胞的谷氨酸诱发电流都有明显的抑制作用,罗哌卡因的抑制作用明显高于布比卡因。AIM： To observe and compare the effects of Ropivacaine and Bupivacaine on glutamate-evoked currents in cultured mt hippocampus neurons. METHODS： Rat hippocampal neurons were dissociated and cultured. Glutamate-evoked currents were recorded by whole cell patch clamp recording. Effects of Ropivacaine and Bupivacaine on glutamate-evoked currents were observed. Drugs were given by pressure ejection or applicated in the bath. RESULTS： Glutamate （100 mmol·L^-1） can activate inward currents in cultured mt this currents could be locked DNQX. At concentrations of hippocampus neurons and by non-NMDA antagonists 10, 50, 100 mmol·L^-1,both Bupivaeaine and Ropivaeaine could obviously decrease glutamate-evoked currents in cultured rat hippocampus neurons. At higher concentration of 50 and 100 mmol·L^-1, the reduced amplitude of glutamate-evoked currents by Ropivacaine was larger than that of by Bupivacaine（ P〈0.01 ）. CONCLUSION： Ropivacaine and Bupivacaine have obviously inhibition effect on glutamateevoked currents in cultured rat hippocampus neurons. The inhibition effect of Ropivacaine on glutamate-evoked currents is stronger than that of Bupivacaine.

关 键 词：布比卡因 罗哌卡因 膜片钳 谷氨酸 海马 

分 类 号：R645.1[医药卫生—外科学]