抗CD28抗体协同刺激增强抗CD3抗体体外激活T淋巴细胞并降低TGF-β的表达  

Anti-CD28 Antibody Costimulation Enhances Anti-CD3 antibod Activating T Cells and Lowering TGF-β Expression in Vitro

作　　者：骆群[1] 吕明[2] 于鸣[2] 黎燕[2] 

机构地区：[1]解放军总医院输血科,北京100853 [2]军事医学科学院基础医学研究所免疫室,北京100850

出　　处：《中国实验血液学杂志》2006年第3期547-551,共5页Journal of Experimental Hematology

摘　　要：为了探讨体外激活T淋巴细胞的方法及活化后T淋巴细胞的免疫学特征,利用抗CD3、抗CD28抗体在体外刺激健康人外周血单个核细胞PBMNC,检测淋巴细胞转化,CD8+CD25+细胞比例和肿瘤生长因子β(TGF-β)表达等相关免疫学指标。结果表明,在抗CD28抗体的协同作用下,抗CD3抗体的刺激活性明显提高,CD8+CD25+细胞比例明显增加,TGF-β的表达下调。结论:抗CD28抗体协同刺激可以提供第二信号,使T淋巴细胞充分活化。In order to study how to activate T cells and their immunological characteristics, the anti-CD3 and anti-CD28 McAbs were used to stimulate PBMNC, then their related immunological changes, such as lymphocyte transformarion function, the percentage of CD8^＋ CD25^＋ cells and TGF-β expression were delected by lymplocyte transformation assay, flow cytometry and RT-PCR respectively, The results showed that in costimularion with anti-CD28 antibody srimulathetion, the activity of anti-CD3 antibody was significantly enchanced, the ratio of CD8^＋ CD25^＋ cells of T cells was obviously increared, while TGF-β expression was down-regulated. It was concluded that the anti-CD28 antibody costimularion could provide srimulatory signal Ⅱ, which make T cells more active, while the expression of TGF-β significantly down-regulated,

关 键 词：抗CD3抗体 抗CD28抗体 TGF-Β T细胞 

分 类 号：R392.1[医药卫生—免疫学]