东北地区高毒力苏云金芽孢杆菌HB-3菌株生物学特性研究  

Biological characteristics of highly toxic Bt strains from northeastern China

作　　者：徐宜宏[1] 纪明山[1] 杨春喜[1] 张弘[2] 于敬沂[3] 

机构地区：[1]沈阳农业大学植物保护学院农药科学实验室,沈阳110161 [2]沈阳化工研究院,沈阳110021 [3]辽宁出入境检验检疫局,大连116001

出　　处：《植物保护》2006年第3期43-46,共4页Plant Protection

基　　金：辽宁省"十五"攻关项目(2001208001)

摘　　要：从自然死亡的玉米螟幼虫体内分离获得一株对玉米螟高毒的苏云金芽孢杆菌,编号为HB-3。该菌株产生菱形和方形两种晶体蛋白。纯化晶体及孢晶混合物的毒力测定结果表明,该菌株对玉米螟具有较高毒力,LC50分别为5.82μg/mL和5.94μg/mL,对尖音库蚊也有一定毒力,LC50分别为16.59μg/mL和17.50μg/mL。生理生化特性研究结果表明,HB-3与已知的蜡螟亚种(Bacillus thuringiensissubsp.galleriae)基本一致,血清型为H5ab。晶体蛋白的SDS-PAGE分析表明,HB-3菌株晶体蛋白至少由分子量为133 kDa、65 kDa4、5 kDa和23 kDa的4种多肽组成。A new strain of Bacillus thuringiensis numbered as HB-3 that was highly toxic to Ostrinia furnacalis Guenee was isolated from the dead larvae of Ostrinia furnacalis Guenee. It produced bipyramid-like and squareneas-like erystals, Bioassay results showed that its crystal and the mixture of spore and parasporal crystal possessed very high toxicity to Ostrinia furnacalis Guenee, with LC50 as 5. 82μg/mL and 5. 94μg/mL, respectively. Meanwhile, they also had steady toxicity to Culecr pipiens Linnaeus, with LC50 as 16. 59 μg/mL and 17. 50 μg/mL, respectively. It is accordant with B. thuringiensis subsp, galleriae in hiochemical reactions, and its serotype belongs to H5ah. Its parasporal crystal consists of four kinds of proteins, i.e. 133 kDa, 65 kDa, 45 kDa and 23 kDa as separated by SDS-PAGE.

关 键 词：苏云金芽孢杆菌 晶体蛋白 毒力测定 生物学 

分 类 号：S476.11[农业科学—农业昆虫与害虫防治]