活性人工骨填充修复兔涂层多孔钛周围骨缺损的生物固定效果  

Biomechanic results of PLGA/TCP scaffold with bovine BMP in treating peri-porous-titanium bone defects in femur of adult rabbits

作　　者：彭磊[1] 胡蕴玉[2] 徐华梓[1] 王臻[2] 孟国林[2] 孙峥[2] 常琪[2] 

机构地区：[1]温州医学院附属第二医院骨科,325027 [2]第四军医大学附属西京医院全军骨科研究所

出　　处：《中华创伤杂志》2006年第6期447-450,共4页Chinese Journal of Trauma

基　　金：国家自然科学基金(30400503)

摘　　要：目的评估骨形态发生蛋白(BMP)复合仿生人工骨三维多孔框架材料[聚(乳酸-羟基乙酸)共聚物(PLGA)/磷酸三钙(TCP)]修复多孔钛周围骨缺损的可行性。方法将仿生人工骨与提取的BMP复合制备活性人工骨材料。建立修复兔股骨下端多孔钛周围骨缺损模型,应用扫描电镜、能谱分析及生物力学研究该材料的生物固定效果及其特点。结果术后6周,实验组样品孔隙内的能谱图上Ca、P元素的峰已经明显。12周时,实验组样品孔隙内部及其周围的Ca、P含量高于对照组(P＜0．05)。生物力学测定结果显示,所有样品的剪切应力都随时间延长而增大。其中在6周、12周,实验组多孔钛在所有时相点均表现出了比对照组高得多的剪切力(P＜0．05)。结论应用BMP复合PLGA/TCP材料修复假体周围骨缺损,早期能够更好地形成新骨,促进假体生物固定。说明BMP早期骨诱导作用在假体生物固定方面至关重要,具有一定的临床应用潜力。Objective To evaluate the feasibility of composite material of bone morphogenetic protein （BMP） and PLGA/TCP in repair of peri-porous-titanium bone defects in adult rabbits. Methods The composite of PLGA/TCP scaffold with bovine BMP was made and implanted in distal bone defects peri-porous-titanium in femur of adult rabbits. The effect of BMP with PLGA/TCP on peri-prosthesis was evaluated by means of scanning electron microscope （SEM）, energy dispersive X-ray analysis （EDX） and biomechanics. Results BMP with PLGA/TCP showed obvious peak of Ca and P of EDX in the pores of titanium in experiment group six weeks after operation and higher than that in control group （ P 〈 0.05）. Push-out test demonstrated that the bonding strength between the composite of HA coating/ porous titanium and bone was increased significantly with time （ P 〈 0.05 ）. In experiment group, at 6 and 12 weeks, peri-porous-titanium had higher shearing force compared with control group （P 〈0.05 ）. Conclusion BMP loaded with PLGA/TCP is a promising bone graft for bone defects in revision arthroplasty, as indicates that bone induction of BMP plays an important role in biological stabilizaiton.

关 键 词：骨形态发生蛋白类 关节成形术 骨缺损 

分 类 号：R687[医药卫生—骨科学]