神经型一氧化氮合酶与血红素氧合酶-2在应激后大鼠结肠的表达  

EXPRESSION OF NEURONAL NITRIC OXIDE SYNTHASE AND HEME OXYGENASE-2 IN COLON AFTER STRESS IN RATS

作　　者：陆国明[1] 章明[2] 朱有法[3] 凌树才[2] 王启志[2] 李玉梅[4] 

机构地区：[1]嘉兴学院医学院解剖学教研室,嘉兴314001 [2]浙江大学医学院解剖学教研室 [3]浙江大学医学院病理学教研室,杭州310031 [4]武警浙江省总队医院消化科,嘉兴314000

出　　处：《解剖学报》2006年第3期340-345,共6页Acta Anatomica Sinica

基　　金：浙江省科技厅资助项目(G20030081);浙江省嘉兴市科技计划资助项目(20031061;20041041)

摘　　要：目的探讨神经型一氧化氮合酶(nNOS)与血红素氧合酶-2(HO-2)在应激后大鼠结肠的表达。方法采用水浸-束缚应激(WRS)动物模型,用免疫组织化学SABC法检测nNOS和HO-2在大鼠结肠中的表达,并通过图像分析系统进行定量测定。结果对照组大鼠nNOS主要表达于结肠黏膜下神经丛和肌间神经丛的神经元,HO-2主要表达于结肠黏膜固有层黏膜肌、肌层环行肌及黏膜下层的血管内皮和平滑肌。应激组黏膜下神经丛和肌间神经丛的nNOS阳性神经元的平均灰度值较对照组明显增加,阳性神经元的平均数高于对照组,且在黏膜上皮细胞、固有层淋巴细胞也有nNOS表达;应激组HO-2阳性黏膜肌的平均灰度值较对照组增加,环行肌阳性单位(PU)明显高于对照组,在部分大肠腺也有HO-2表达。与应激组比较,应激+L-NAME组的nNOS阳性神经元的平均灰度值减少,阳性神经元的平均数下降,应激+ZnPP组HO-2阳性黏膜肌平均灰度值减少,环行肌PU下降。结论一氧化氮(NO)和一氧化碳(CO)均是结肠重要的气体信号分子和神经递质,两者在应激所致的结肠功能失调中可能具有协同作用。Objective To investigate the expression of neuronal nitric oxide synthase （nNOS） and heme oxygenase-2 （HO-2） in colon after stress in rats. Methods Water immersion-restraint stress（WRS） model was performed, The expressions of nNOS and HO-2 in colon of rats were examined by immunohistochemical staining and analysed by image analysis system. Results In control group rat, nNOS immune-positive substance mostly expressed in neurons of submucous plexus and myenteric plexus and HO-2 mostly expressed in lamina propria mucosal muscles of colon mucosa, circular muscles of colon and the endothelium and smooth muscle cells of submucosal bloodvessels. Compared with control group rat, the average gray value of nNOS positive neurons in submucous plexus and myenteric plexus and the average number of nNOS positive neurons increased significantly, the average gray value of HO-2 positive mucosal muscles and the positive unit （PU） of circular muscles increased in stress group rat, moreover, nNOS expression in mucosal epithelial cells and lamina propria lymphocytes and HO-2 expression in some large intestinal glands were observed. Compared with stress group rat, the average gray value of nNOS positive neurons in submucous plexus and myenteric plexus and the average number of nNOS positive neurons decreased in stress ＋ L-NAME group, the average gray value of HO-2 positive mucosal muscles and the PU of circular muscles decreased in stress ＋ ZnPP group. Conclusion Both nitric oxide（NO） and carbon monoxide（CO） are important gaseous signal molecules and neurotransmitters in colon of rats and may play a cooperative role in colonic functional disorder induced by WRS.

关 键 词：神经型一氧化氮合酶 血红素氧合酶-2 应激 结肠 免疫组织化学 大鼠 

分 类 号：R333.3[医药卫生—人体生理学]