藏红T褪色分光光度法测定微量肝素  

Spectrophotometric determination of heparin with safranine T

作　　者：徐红[1] 刘绍璞[2] 罗红群[2] 

机构地区：[1]贵阳医学院化学教研室,贵阳550004 [2]西南师范大学环境化学研究所,重庆410715

出　　处：《分析试验室》2006年第6期32-35,共4页Chinese Journal of Analysis Laboratory

摘　　要：在pH 5.02的Britton-Robinson缓冲溶液中,肝素与碱性吩嗪染料藏红T形成离子缔合物,染料发生明显的褪色,体系吸光度的降低与肝素钠质量浓度成正比,0～2μg/mL范围内符合比耳定律,比吸光系数为0.36×10^3 L·g^-1·cm^-1.研究了表面活性剂和共存物质的影响,表明方法选择性好.用于肝素钠注射液效价的测定.A new spectrophotometric method for the determination of trace heparin has been studied, based on the discoloration reaction between heparin and Safranine T in the Britton-Robinson buffer solution at pH 5.02. The reduction in absorbance is directly proportional to the concentration of heparin. The linear range of heparin is from 0 to 50 μg/25 mL. Specific absorptivity of the method is 0.36×10^3 L·g^-1·cm^-1 The effects of some surfactants and coexisting substances on the reaction have been investigated and the results show that the selectivity of the method is good. The method has been applied to determine the potency of heparin sodium injections with satisfactory results.

关 键 词：分光光度法 肝素 藏红T 

分 类 号：O657.3[理学—分析化学]