以鸡冠花红为电化学探针线性扫描极谱法测定阿米卡星  

Linear sweep polarographic determination of amikacin with amaranth as electrochemical probe

作　　者：王学亮[1] 

机构地区：[1]菏泽学院化学与化工系,菏泽274015

出　　处：《分析试验室》2006年第6期43-45,共3页Chinese Journal of Analysis Laboratory

摘　　要：在pH 4.0的介质中鸡冠花红在-250 mV（vs.SCE）处有一个良好的二阶导数极谱还原峰,当其与阿米卡星作用后,其还原峰电位基本不变而峰电流明显下降,峰电流的下降值与阿米卡星的质量浓度在0～40.0 mg/L范围内呈良好的线性关系,其线性方程为△ip＂（nA）=-5.48＋72.28ρ（mg/L）,相关系数r=0.9996,检出限为1.0 mg/L.优化了线性扫描极谱法测定阿米卡星的试验条件,并将该方法应用于市售药物中阿米卡星的测定.Amaranth has a sensitive second order derivative polarographic reduction peak in Britton-Robinson （B-R） buffer solution （pH 4.0）. When adding amikacin to above solution, the reduction peak current for amaranth decreased linearly with the concentration of amikacin in the range of 0 ～ 40.0 mg/L. Regression equation is Aip＂（nA） = -5.48 ＋ 72.28c （mg/L）, and correlation coefficient （r） is 0. 9996. The detection limit is 1.0 mg/L. The determination conditions for the concentration of amikacin by linear sweep polarography were optimized. The method was used to determine the content of amikacin commercial-available with satisfactory results.

关 键 词：鸡冠花红 阿米卡星 极谱法 

分 类 号：O657.1[理学—分析化学]