反相高效液相色谱法测定糠酸、糠醇和糠醛  

Determination of furoic acid,furfuryl alcohol and furfural by reversed-phase high performance liquid chromatography

作　　者：石起增[1] 杨光瑞[1] 刘巧茹[1] 

机构地区：[1]河南师范大学化学与环境科学学院河南省环境污染控制重点实验室,新乡453007

出　　处：《分析试验室》2006年第6期73-74,共2页Chinese Journal of Analysis Laboratory

基　　金：河南省新乡市科技发展计划资助项目(04G115)

摘　　要：提出了反相高效液相色谱法(HPLC)同时测定糠酸、糠醇和糠醛的新方法,采用C18色谱柱,流动相为V(乙腈)∶V(水)=50∶50,流速为1.0 mL/min;检测波长为220 nm;柱温为25℃。在4 min内可以将3种组分完全分离。糠酸、糠醇、糠醛检出限(S/N=3)分别为:0.101、0.042、0.406 mg/L。平均回收率分别为101.4%、98.5%、95.3%。相对标准偏差(RSD)分别为0.87%、0.66%、0.43%。A new reversed phase high performance liquid chromatographic method was established for the derermination of furoic acid, furfuryl alcohol and furfural, furoic acid, furfuryl alcohol and furfural were separated on a C18 column with aeetonitrile and water （the ratio of volume is 50：50） as mobile phase at the flow rate of 1.0 mL/min. The column temperature was controlled at 25 ℃and the wavelength of detector was set at 220 nm. The component were separated in four minues. The detection limits of furoic acid, furfuryl alcohol and furfural were 0. 101,0.042, 0.406 mg/L, respectively. The average recoveries of furoic acid, furfuryl alcohol and furfural were 101.4%, 98.5%, 95.3%. RSD were 0.87%, 0.66%, 0.43%.

关 键 词：反相高效液相色谱法 糠酸 糠醇 糠醛 

分 类 号：O657.7[理学—分析化学]