河南省鸡致病性大肠杆菌耐庆大霉素质粒的检测分析  

Study on Plasmids Resistance to Gentamycin of Avian Pathogenic Escherichia Coli Isolates from Henan Province

作　　者：王永芬[1] 席磊 李立家[1] 

机构地区：[1]武汉大学生命科学学院,湖北武汉430072 [2]郑州牧业工程高等专科学院,河南郑州450011

出　　处：《中国家禽》2006年第10期18-20,共3页China Poultry

基　　金：河南省自然科学基金项目(0411032000)

摘　　要：通过对12株100%耐庆大霉素的多重耐药菌株的质粒提取,电泳检测和转化试验,成功获得了2株由质粒介导的耐庆大霉素的多重耐药菌株。结果表明:同一质粒可编码一个至数个耐药性基因,不同质粒可以携带相同的耐药性基因,并且这些质粒可以传递多种耐药性。证明了菌株对庆大霉素的耐药性是由耐药性质粒介导的,细菌的抗药性与耐药性质粒的转移有很大关系。We succeeded to gain two muhiple-resistance isolates from twelve Avian pathogenic Escherichia Coli islates with hundred-percent resistance to gentamycin by plasmid electrophoresis detection and transformed experiment.The result showed that a plasmid can encode single or several resistance genes,and different plasmid also can have same resistance genes.These plasmids can transform multiple resistance.It suggested that the resistance to gentamycin of Escherichia coli isolate was mediated by resistance plasmid.The resistance of bacteria has close relation with the transfer of resistance plasmid.

关 键 词：鸡致病性大肠杆菌 庆大霉素 耐药性质粒 

分 类 号：S858.28[农业科学—临床兽医学] TQ465.2[农业科学—兽医学]