当归多糖硫酸酯的合成及其抗小鼠L6565逆转录病毒的作用  

Sulfation of polysaccharides from Angelica sinensis and its effect against L6565 murine retrovirus

作　　者：杨铁虹[1] 贾敏[1] 姚秀娟[1] 孟嘉[1] 孟静茹[1] 梅其炳[1] 

机构地区：[1]第四军医大学药理学教研室,西安710032

出　　处：《中国新药杂志》2006年第10期783-786,共4页Chinese Journal of New Drugs

基　　金：陕西省中医管理局资助项目(0347)

摘　　要：目的:对当归多糖进行硫酸酯化修饰,并观察当归多糖硫酸酯(APS)抗小鼠L6565逆转录病毒的作用,以探讨氯磺酸对当归多糖硫酸酯化过程的影响及APS治疗艾滋病的可能性。方法:采用氯磺酸-吡啶法进行硫酸化修饰;硫酸钡比浊法测定修饰物的硫含量;用小鼠L6565白血病病毒感染出生24 h内的乳鼠建立小鼠艾滋病模型;以RT-PCR法测定血浆病毒载量;流式细胞术测定CD_4^+和CD_8^+细胞的百分数,并计算CD_4^+/CD_8^+细胞的比值。结果:得到8种不同取代度的APS(APS-1～8),硫含量为9．37%～17．14%,收率为73．7%～88．4%。与正常对照组相比,模型组小鼠血浆病毒载量较高,CD_4^+细胞百分率显著降低;与模型组相比,APS-4组在10和30 mg·kg^(-1)剂量下血浆病毒载量显著降低,CD_4^+细胞百分率和CD_4^+/CD_8^+比值显著升高。结论:提高氯磺酸的比例,可以提高产物的硫含量,但收率降低。APS不仅具有一定的抗病毒作用,而且还具有一定免疫功能调节作用,提示其对艾滋病可能有一定的治疗作用。Objective： To investigate the effect of chlorosulfonic acids on the synthesis of Angelica polysaccharide sulfate （APS） and assess the APS activities against L6565 murine retrovirus. Methods： Angelica sinensis polysaecharide was sulfated using a chlorosulfonic acid-pyridine method; the ester sulfate level was turbidimetrically measured in barium sulfate. An AIDS model of mice was set up by infecting infant mice with L6565 murine leukemia virus. The viral load in plasma was measured using a quantitative RT-PCR method. The percentages of CD4^＋ and CD8^＋ lymphocytes were count using a floweytometric method to determine the ratio of CD4^＋/CD8^＋. Results： Eight derivatives of APS with different sulfur content （9.37% - 17.14% ） were synthesized. The yield was 73.7% - 88.4%. Compared with the model mice, the mice treated with APS 10 and 30 mg.kg^-1 had significant reduction of the viral load in plasma, and significant increase of the percentage of CD4^＋ lymphocyte and the ratio of CD4^＋/CD8^＋. APS showed activities against the retrovirus as well as the immunomodulative effect. Conclusion： The ratio of chlorosulfonic acid in the initial reactants was positively proportional to the sulfur level in the final products, but the inversely to the final yields. APS is warranted to further test the effect against AIDS.

关 键 词：当归多糖 多糖硫酸酯 逆转录病毒 病毒载量 

分 类 号：R285.5[医药卫生—中药学] R972.4[医药卫生—中医学]