HPLC法测定人血浆中盐酸阿比朵尔浓度  

Quantification of abidol hydrochloride in human plasma by HPLC

作　　者：何清[1] 封宇飞[2] 李可欣[2] 孙春华[2] 

机构地区：[1]卫生部北京医院普外科,北京100730 [2]卫生部北京医院临床药理室,北京100730

出　　处：《中国新药杂志》2006年第10期809-811,共3页Chinese Journal of New Drugs

摘　　要：目的:建立盐酸阿比朵尔血药浓度测定方法。方法:采用高效液相色谱紫外检测法测定盐酸阿比朵尔的血药浓度,检测波长315 nm。血样加入乙腈沉淀;色谱柱:Alltech Apollo C_(18)(250 mm×4．6 mm,5μm);流动相A为0．1%三乙胺水溶液(加磷酸调pH值至2．0),流动相B为乙腈。梯度洗脱程序:A与B的体积比变化为52．5: 47．5(0～5．5 min),52．5:47．5～10:90(5．5～6．5 min),10:90～0:100(6．5～6．6 min),0:100(6．6～8 min),0:100～52．5:47．5(8～8．5 min)。流速:1．0 mL·min^(-1)。结果:在此条件下,盐酸阿比朵尔与血浆中其他成分分离完全,线性范围为5～1280 ng·mL^(-1),最低检测浓度为5 ng·mL^(-1),相对回收率为93．6%～99．0%,日内日间精密度RSD为0．5%～3．6%。结论:高效液相色谱紫外法稳定、灵敏、可靠,可用于人体血浆中盐酸阿比朵尔浓度的测定。Objective： To establish a HPLC method to determine abidol hydrochloride in human plasma. Methods： The HPLC was performed with an Alhech Apollo C18 column （250 mm × 4.6 mm,5μm）, a gradient elution procedure consisted of eluate A （0. 1% acetic acid pH2.0） and B （ acetonitrile） with a flow rate of 1.0 mL.min ＇ and UV detection at 315 nm. The gradient elution procedure was programmed as 0-5.5min 52.5：47.5; 5.5 -6.5 min52.5：47.5 -10：90; 6.5 -6.6 min 10：90 - 0：100 ; 6.6 - 8 min 0：100 and 8 - 8.5 min 0：100 - 52.5： 47.5. Results ： The calibrated linear curve of abidol hydrochloride concentration was in a range of 5 - 1 280 ng. mL^-1. The detection limit was 5 ng.mL^-1, and relative recovery rate of 93.6% - 99.0%. The within-day and between-day RSD was 0.5% -3.6% o Conclusion： The accurate and reproducible HPLC method is used to quantify abidol hydrochloride in human plasma.

关 键 词：盐酸阿比朵尔 高效液相色谱法 血药浓度 

分 类 号：R978.7[医药卫生—药品] R917.1[医药卫生—药学]