红树林耐盐相关基因转化水稻的研究  

Transformed Rice with Salt Tolerance-related Genes of Bruguiera sexangula by Agrobacterium Meditation

作　　者：张文惠[1] 林涛[2] 张红心[2] 陈亮[2] 山仑[1] 

机构地区：[1]西北农林科技大学生命科学学院,陕西杨陵712100 [2]厦门大学生命科学学院细胞生物学与肿瘤细胞工程教育部重点实验室,福建厦门361005

出　　处：《西北植物学报》2006年第6期1105-1109,共5页Acta Botanica Boreali-Occidentalia Sinica

基　　金：中国科学院知识创新工程重要方向项目(KZCX3-SW-444);教育部重点项目(01102)

摘　　要：运用农杆菌介导法将红树林耐盐相关基因mangrin转入粳稻品种‘日本晴’中,通过GUS基因检测愈伤组织转化率,确定农杆菌菌液浓度OD600为0.5,浸染时间30 min,共培养时间3 d为最佳转化体系;经潮霉菌筛选,获得抗性再生植株.通过PCR扩增检测、Southern blot分析和GUS基因活性检测,结果表明,mangrin基因整合到再生水稻的染色体DNA上.耐盐性测定结果表明,转基因植株在200 mmol/L NaCl胁迫下,成活率保持在83.3%,株高增长20%～40%,mangrin基因能提高转基因水稻对盐胁迫的抗性.Salt tolerance-related genes in P, ruguiera sexangula were transformed into Oryza sativa suhsp. japonica cv. Nipponbare,a keng-rice variety. The optimal system of the transformation was found by detecting the callus-transformation rate with GUS gene to be the one with the agro-bacterium concentration set at OD600 0.5,the infection time set at 30 minutes and the culture time set at 3 days and then the regeneration plantlets were obtained by Hyg screening, mangrin gene was integrated in the DNA of the regeneration plantlets by PCR amplification,Southern blotting and GUS gene activity detection. The testing of the salt tolerance indicated that under the stress resulting from 200 mmol/L NaCl the transgenic plants maintained a survival rate of 83.3% and increased their heights by 20%-40% and thus mangrin gene could raise the resistance of the transformed rice to salt stress.

关 键 词：红树林 耐盐相关基因 水稻 转化 

分 类 号：Q785[生物学—分子生物学] Q789