血小板源性生长因子对大鼠内侧侧副韧带愈合的影响  

EFFECT OF PLATELET DERIVED GROWTH FACTOR BB ON HEALING OF MEDIAL COLLATERAL LIGAMENT IN RATS

作　　者：任逸众[1] 李文琪[1] 刘晓民[1] 

机构地区：[1]内蒙古医学院第二附属医院关节外科,呼和浩特010030

出　　处：《中国修复重建外科杂志》2006年第6期616-619,共4页Chinese Journal of Reparative and Reconstructive Surgery

摘　　要：目的观察局部应用血小板源性生长因子B链(plateledderivedgrowthfactorBB,PDGF-BB)对韧带愈合质量的影响。方法成年雄性Wistar大鼠48只,随机分为实验组(A组)与对照组(B组),每组24只。制备右膝关节内侧侧副韧带(medicalcollateralligament,MCL)断裂模型,A组每只大鼠在伤口局部注入5μgPDGF-BB,然后用9-0可吸收缝线行褥式缝合;B组伤口直接缝合。术后2周,切取韧带行组织学、苦味酸-天狼猩红染色和免疫组织化学观察。结果A组韧带成纤维细胞数量为213.44±15.32,B组为180.42±12.78,二者差异有统计学意义(P<0.01),且A组细胞较B组更成熟,排列更规则。A组胶原类型、含量及其交联程度均较B组有改善(P<0.01)。结论PDGF-BB对韧带的愈合具有促进作用。Objective To examine an effect of the locally-used platelet derived growth factor-BB （PDGF-BB） on the healing of the medial collateral ligament （MCL） in the knee joints of rats. Methods Forty-eight rats were equally randomly divided into 2 groups： the experimental group （group A） and the control group （group B）. MCL of all the rats were ruptured to establish the wound models. In group A, 5 μg of PDGF-BB was locally injected in the wound of each rat and then the wound was sutured; but in group B, the wound was only sutured. After 2 weeks, histological evaluations were performed to determine whether PDGF-BB could promote the healing of MCL. Results There were significantly more fibroblasts formed during the ligament healing process in group A than in group B （213.44±15.32 vs. 180.42±12.78, P〈0.01）. The fibroblasts were more mature and more regularly-arranged in group A than in group B. The type, content, and crosslink of the collagen were improved to a greater extent in group A than in group B （P〈0. 01）. Conclusion PDGF can promote the healing of the injured ligament.

关 键 词：内侧侧副韧带 血小板源性生长因子 愈合 大鼠 

分 类 号：R687.2[医药卫生—骨科学]