L-NAME和Leupeptin对庆大霉素所致耳蜗毛细胞损伤协同保护作用的观察  

Synergetic Protective Effects of L-N^G-nitroarginine methylester and Leupeptin on Damaged Cochlear Hair Cell Caused by Gentamin

作　　者：田媛[1] 姜学钧[2] 

机构地区：[1]中国医科大学附属第二医院耳鼻咽喉科,辽宁沈阳110001 [2]中国医科大学附属第一医院耳鼻咽喉科,辽宁沈阳110001

出　　处：《解剖科学进展》2006年第2期109-112,共4页Progress of Anatomical Sciences

摘　　要：目的观察一氧化氮合酶抑制剂[L-NG-硝基精氨酸甲酯(L-NG-n itroargin ine m ethylester,L-NAME)]和钙蛋白酶抑制剂[leupeptin]对庆大霉素(gentam ic in,GM)所致耳蜗毛细胞损伤的协同防护作用。方法将20只出生后3~5天的W istar大鼠(40耳)平均分成5组,1组为正常对照,其余4组在施加GM的同时单独或联合应用不同的保护因素(L-NAME和leupeptin)。通过耳蜗器官离体培养,对基底膜铺片行TR ITC-Phalloid in荧光染色,观察各组耳蜗毛细胞的生长情况并比较存活率。结果GM组毛细胞存活极少,L-NAME和leupeptin单独应用时毛细胞存活率明显高于GM组(P<0.01),二者联合应用时耳蜗毛细胞存活率显著高于L-NAME和leupeptin单独用药组(P<0.01)。结论L-NAME和leupeptin对庆大霉素耳毒性所致毛细胞损伤具有较好的协同防护作用。Objective To investigate the synergetic protective effect of a nitric oxide synthase （NOS） in- hibitor-L-N^G-nitroarginind methylester （L-NAME） combined with a calpain inhibitor-leupeptin on cochlear hair cells damaged by gentamicin（GM）. Methods 20 Wistar rats （40 ears） of post-natal 3 -5 days were divided equally into 5 groups： normal control, GM, L-NAME and leupeptin alone or together. The survival of cochlear hair ceil in five groups was investigated by means of cochlear organotypic culture, cochlear surface preparation was stained with TRITC-PhaUoidin fluorescein. Results Less cochlear hair cells were seen in GM, cell survival percentage in group treated with L-NAME or leupeptin alone was mere than that in GM group （ P 〈 0. 01 ）. Combination of L-NAME and leupeptin showed a higher survival percentage of cochlear hair cells than those with L-NAME （ P 〈 0.01 ） or leupeptin （P 〈 0. 01） alone. Conclusion The combination of L-NAME and leupeptin has more effective protection than alone for the cochlear hair ceils from GM ototoxity.

关 键 词：L-NG-硝基精氨酸甲酯 LEUPEPTIN 耳蜗 毛细胞 庆大霉素 

分 类 号：R764.3[医药卫生—耳鼻咽喉科]