大鼠再生肝中hsbp1、hsf1、hsf2、hsp70表达水平改变的分析  

ANALYSIS OF CHANGES ABOUT hsbp1,hsf1,hsf2 AND hsp70′S EXPRESSION LEVELS IN RAT′S REGENERATING LIVER

作　　者：苏丽娟[1] 常翠芳[2] 韩鸿鹏[2] 马辉[2] 徐存拴 

机构地区：[1]中国海洋大学,青岛266003 [2]河南师范大学,新乡453007

出　　处：《分子细胞生物学报》2006年第3期258-264,共7页Journal of Molecular Cell Biology

基　　金：国家自然科学基金资助项目(批准号:30340042);河南省动物学重点学科资助项目(批准号:KSF030018)

摘　　要：在克隆了大鼠热休克因子结合蛋白1基因(hsbp1)全长cDNA基础上,进一步分析它在肝再生中作用。用SD纯系大鼠为材料,按Higgens等方法建立大鼠部分肝切除(PH)模型;用原位杂交等方法分析hsbp1在肝再生中表达变化;用基因表达谱芯片分析hsbp1、hsf1、hsf2和hsp70在肝再生中表达变化。原位杂交和基因表达谱芯片分析表明,PH后6h和66-144h,hsbp1表达发生了有意义上调;8-16h,hsf1表达发生了有意义上调;2-16h,hsf2表达发生了有意义上调;0.5-24h,hsp70表达发生了有意义上调。假手术(只打开腹腔和翻动肝叶,但不进行部分肝切除)后0.5-2h,hsbp1表达发生了有意义下调;8-16h,hsf1表达发生了有意义上调;0-144h,hsf2未发生有意义表达变化;0.5-30h,hsp70表达发生了有意义上调。根据实验结果推测,PH后hsbp1表达上调可增加细胞内HSBP1量,促进生长、发育、分化相关基因表达和再生肝的组织结构功能重建;(假)手术后hsbp1表达下调可减少细胞内HSBP1量,有利于HSF1上调hsp70表达,提高机体和肝脏抗损伤能力。Heat shock factor binding protein 1 （HSBP1）,a recently discovered protein,weakens and blocks transcription of the heat shock protein 70 （HSP70） gene when binding to HSF1 ,but HSBP1 can promote cell growth,cell development and cell differentiation when binding to HSF2. Partial hepatectomy （PH） in rat creates injury stimulation and induces liver regeneration. How does hsbp1 coordinate two processes sequently is extremely interesting. This paper,based on cloning the full-length cDNA of hsbp1 in rat,applied in situ hybridration and Rat Genome 230 2.0 Array to analyze hsbp1,hsfl,hsf2 and hspTO expression in liver after PH and sham-operation. The results indicated that the hsbp1 expression level was down-regulated meaningfully at 0.5-2h and up-regulated meaningfully at 8-16h after sham-operation,while hsf2 expression level did not meaningfully change at 0-144h after sham-operation, hsbp1 expression level was up-regulated meaningfully at 6h and 66-144h,and hsfl at 8-16h,hsf2 at 2-16h,hsp70 at 0.5-24h after PH. Our data suggested that up-regulated expression of the hsp70 at 0.5-12h after sham-operation was controlled by intracellular HSFl,and then controlled by hsbp1 down-regulated at 0.5-2h and hsfl up-regulated at 8-16h. In the early phase of liver regeneration in rats,hsbp1 and hsf2 expression levels were up-regulated, which promoted cell proliferation through HSBP1 and HSF2 up-regulating,upa activating,c-jun enhancing, intracellular matrix （ECM） degradation, activating the hepatocyte-like growth factor （HGF） etc. In the late phase of liver regeneration （66-144h）, hsbp1 expression level was up-regulated,which promoted reconstruction of liver structure and recovery of liver function through HSBP1 inhibiting hsp70 expression,up-regulating genes related to growth, development, differentiation. In conclusion,down-regulating of hsbp1 contributed to interac- tion between HSF1 and HSE,increased hsp70 expression and enhanced anti-injured capacity of liver and rats. HSBP1 and HSF2 activated the genes re

关 键 词：大鼠部分肝切除(PH) 肝再生 热休克因子结合蛋白1基因(hsbp1) 基因表达谱芯片分析 原位杂交 

分 类 号：Q819[生物学—生物工程]