五种静脉麻醉药对人体中性白细胞吞噬功能的影响  

Effects of intravenous anesthetics on the phagocytosis of human neutrophils

作　　者：刘玉莲[1] 张卓然[2] 刘静[1] 于永群[1] 徐廷万[1] 陈敏茹[1] 刘雁[1] 

机构地区：[1]大连市中心医院麻醉科,116033 [2]大连医科大学微生物教研室

出　　处：《中华麻醉学杂志》1996年第4期177-179,共3页Chinese Journal of Anesthesiology

摘　　要：取健康人外周肝素抗凝血，用细胞分离液分离出中性白细胞（ＰＭＮ）以１×１０＾６／ｍｌ浓度与氟哌利多、咪唑安定、芬太尼、乙咪酯、羟丁酸钠麻醉药物四种不同剂量的稀释液，对照组为生理盐水混匀分别置于２４孔培养板内孵育。孵育后１０分钟、３０分钟、２小时、２４小时又分别加等量６￣９亿／ｍｌ白色葡萄球菌液再孵育４５分钟后涂片镜检。计算各种药物、浓度、各时间的ＰＭＮ吞噬率。结果表明。The effects of intravenous anesthetics on phagocytosis of human neutrophils were observed in vitro. 1×10~6/ml of neutrophils, isolated from heparinized venous blood of healthy human,was mixed at equivalent volume with normal saline (as control), four different concentrations (clinical doses) of droperidoI, midazolam, fentanyl,etomidate or sodium hydroxybutyrate respectively. All mixtures were incubated in each of four 24-pore culture plates. The equivalent volumes of staphylococci 6～9×10~9/ml were added to these mixtures, 10 mins, 30 mins, 2hs or 24hs after incubation individually. The successive incubations lasted 45 mins,and the neutrophil phagoeytosis rate (NPR) was calculated. The results showed that NPR was correlated negatively with the concentration of each anesthetic mentioned above. but was related to its acting-duration. It is demonstrated that these intravenous anesthetics may reduce the phagoeytosis of neutrophils in a dose-related way,therefore we suggest that antibiotics or antineoplastics should be used in combination with these anesthetics during operation.

关 键 词：静脉麻醉药 中性白细胞 吞噬功能 

分 类 号：R971.2[医药卫生—药品]