骨髓基质细胞系BMSC1自发分泌多种细胞因子  

Multiple types of cytokines produced constitutionally by murine bone marrow stromal cell line BMSCl

作　　者：刘倩[1] 张平夏[1] 刘祝公[1] 钱晓萍[1] 庞学文[1] 陈慰峰[1] 

机构地区：[1]北京医科大学免疫学系,100083

出　　处：《中华微生物学和免疫学杂志》1996年第2期113-116,共4页Chinese Journal of Microbiology and Immunology

基　　金：中华医学基金会!CMB;国家自然科学基金

摘　　要：骨髓基质细胞（ＢＭＳＣ）及其分泌的细胞因子在造血细胞分化发育过程中起重要作用。应用特异性细胞因子依赖株检测了自建的小鼠骨髓基质细胞株ＢＭＳＣ１的细胞因子分泌情况。实验说明ＢＭ－ＳＣ１细胞能自发分泌高水平的ＩＬ－６，中等水平的ＩＬ－７及较低水平的ＧＭ－ＣＳＦ，未检测到化学趋化因子。此细胞上清对骨髓造血干细胞有明显的促集落形成效应，所形成的集落以ＣＦＵ－ＧＭＭ及ＣＦＵ－ＧＭ为主，其促集落形成作用呈现剂量依赖关系。上述结果表明，骨髓基质细胞能够产生多种类型的细胞因子从而对于细胞骨髓内分化发育成Ｐｒｏ－Ｔ细胞可能起重要作用。A murine bone marrow stromal cell sin (BMSC1)has been established. Without exogenous GM-CSF. Of which , IL-6 was abundant , IL-7 was moderate , and GM-CSF at low level. BMSC1 cells did not produce any detectable chemotactic factor. BMSC1-SN sustained colony unit formation of murine bone marrow cells in a dose dependent fashion. The dominent types of colonies induced by BMSC1-SN were mixed granulocyte/monocyte/megakaryocyte colonies (CFU-GMM ) and granulocyte/macrophage colonies ( CFU-GM ) . The cytokines secreted by BMSC 1 might play an important role in T cell development in bone marrow. These cytokines might be useful in analysis of the mechanism of T cell development in bone mar- row and in the study of the network regulation of autocrined cytokines.

关 键 词：骨髓基质细胞 分泌 细胞因子 T细胞 

分 类 号：R331.22[医药卫生—人体生理学]