反相高效液相色谱法测定依普黄酮的含量  

Determination of Ipriflavone by RP-HPLC

作　　者：徐松林[1] 邓定安[1] 蔡俊超 

机构地区：[1]中国科学院上海药物研究所

出　　处：《药物分析杂志》1996年第2期81-83,共3页Chinese Journal of Pharmaceutical Analysis

摘　　要：本文应用反相高效液相色谱法，以萘为内标，建立了测定依普黄酮含量的方法，用μＢｏｎｄａｐａｋＣ＿（１８），１０μｍ色谱柱，甲醇－水（７５：２５）为流动相，Ｍ９９０光电二极管阵列检测器，２４６ｎｍ检测。在０．０１～０．４０ｍｇ／ｍｌ范围内，峰面积比与浓度呈良好的线性关系，ｒ＝０．９９９９。平均回收率为（１００．２±１）％，方法简便、准确、灵敏。A simple. accurate and sensitive method for the determination of ipriflavone was developed. The quantification of ipriflavone was carried out by a reversed-phase HPLC using μBondapak C_(18) column and methanol-water (75 : 25) as the mobile phase. Waters 990 photodiode array detector was used for the detection of eluates at 246nm. Excellent linearity between peak areas and concentrations of ipriflavone was obtained in the concentration range of 0. 01～0. 4 mg/ml. Naphthalene was employed as the internal standard.

关 键 词：依普黄酮 高效液相色谱法 内标法 

分 类 号：R927.2[医药卫生—药学]