Production of 2,3-Butanediol by Klebsiella Pneumoniae Using Glucose and Ammonium Phosphate  

作　　者：秦加阳 肖梓军 马翠卿 谢能中 刘培海 许平 

机构地区：[1]State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, China

出　　处：《Chinese Journal of Chemical Engineering》2006年第1期132-136,共5页中国化学工程学报（英文版）

摘　　要：The production of 2,3-butanediol by Klebsiella pneumoniae from glucose supplemented with different salts was studied. A suitable medium composition was defined by response surface experiments. In a medium containing glu-cose and (NH4)2HPO4, the strain could convert 137.0g of glucose into 52.4g of 2,3-butanediol and 8.4g of acetoin in shaking flasks. The diol yield amounted to 90% of its theoretical value and the productivity was 1—1.5g·L-1·h-1. In fed-batch fermentation, the yield and productivity of diol were further enhanced by maintaining the pH at 6.0. Up to 92.4g of 2,3-butanediol and 13.1g of acetoin per liter were obtained from 215.0g of glucose per liter. The diol yield reached 98% of its theoretical value and the productivity was up to 2.1g·L-1·h-1.The production of 2,3-butanediol by Klebsiella pneumoniae from glucose supplemented with different salts was studied. A suitable medium composition was defined by response surface experiments. In a medium containing glucose and （NH4）2HPO4, the strain could convert 137.0g of glucose into 52.4g of 2,3-butanediol and 8.4g of acetoin in shaking flasks. The diol yield amounted to 90% of its theoretical value and the productivity was 1-1.5g.L^-1.h^-1. In fed-batch fermentation, the yield and productivity of diol were further enhanced by maintaining the pH at 6.0. Up to 92.4g of 2,3-butanediol and 13.1g of acetoin per liter were obtained from 215.0g of glucose per liter. The diol yield reached 98% of its theoretical value and the productivity was up to 2.1g.L^-1.h^-1.

关 键 词：2.3-butanediol producfion optimizafion fed-batch. Klebsiella pneumoniae 

分 类 号：O621.3[理学—有机化学]