高脂膳食和VE对冷暴露人红细胞膜Na，K－ATP酶活性及血浆脂质过氧化的影响  

EFFECT OF DIETARY FAT AND VITAMIN E ON THE CHANGES OF ERYTHROCYTIC MEMBRANE Na,K一ATPase AND LPO IN THE PLASMA OF COLD-EXPOSED HUMAN

作　　者：王枫[1] 陈耀明[1] 董兆申[1] 

机构地区：[1]第四军医大学军队卫生教研室

出　　处：《营养学报》1996年第1期39-43,共5页Acta Nutrimenta Sinica

基　　金：军队"八五"规划课题

摘　　要：本研究用人群实验，探讨了寒冷条件下，高脂膳食和维生素Ｅ对人红细胞膜Ｎａ，Ｋ一ＡＴＰａｓｅ活性和血浆脂质过氧化的影响，分析了酶活性与血浆ＭＤＡ间的关系。结果表明：摄入３５％（产能量比）高脂膳食１５天后，人红细胞膜Ｎａ，Ｋ一ＡＴＰａｓｅ活性显著高于１２％膳食脂肪组（Ｐ＜０．０５），血浆ＭＤＡ含量比实验前略有降低。高脂膳食同时每天补充２００ｍｇ维生素Ｅ组，Ｎａ，Ｋ一ＡＴＰａｓｅ活性比单纯高脂膳食组明显升高（Ｐ＜０．０５），而血浆ＭＤＡ含量则比实验前显著降低（Ｐ＜０．０５）。相关分析表明，各组实验前后酶活性与ＭＤＡ含量问均呈负相关。由此可见，高脂膳食和ＶＥ均能使人红细胞膜Ｎａ，Ｋ一ＡＴＰａｓｅ活性升高；ＶＥ可降低血浆中ＭＤＡ含量。ＭＤＡ与Ｎａ，Ｋ一ＡＴＰａｓｅ呈负相关。推测可能是脂肪和ＶＥ通过保护细胞膜引起了酶活性升高。or the elucidation of the effect of dietary fat and vitamin E on cold一exposed people we investigatetl the activity of erythrocytic(RBC)membr-ane Na,K一ATpase and the level of LPO product MDA in the plasma,60recruits in cold area were randomized into three groups: 12%of calories(LF),35%of calories(HF)and 35%of calorie from fat and oil and200mg/day of Vh(HFE).5ml venous blood were drawn from each personfor the measurement of the RBC membrane Na,K-ATPase activity and the level of MDA in the plasma on the first day of their arrival to the cold area and the fifteenth day after providingthe experimental diets.The ac一tivity of RBC membrane Na,K-ATPase of all groups was obviously incr-eased(LF 0.2103±0.1067/0.3446±0.1439,HF0.2069±0.1328/0.5504±0.2647,HFE 0.1862 ±0.1216/0.7219±0.2190μmol·mg prot-1·h-1. The increment in HFE was the highest and that in HF was higher than that in LF. In the same time,the levcl of MDA was lower than that before the trial especialy in HFE.Negative correlation was found between the Na,K-ATPase acti-vity and the content of plasma MDA,These findings indicated that either high dietary fat or VE could increased the activity af RBC Na,K-ATPase in cold-exposed human and these changcs related to the change of MDA content in the plasma.

关 键 词：冷暴露 高脂膳食 维生素E 红细胞膜 

分 类 号：R339.55[医药卫生—人体生理学] R594.2[医药卫生—基础医学]