锌缺乏对大鼠骨骼肌α－肌动蛋白基因表达的影响  

EFFECT OF ZINC DEFICIENCY NO α一ACTIN GENE EXPRESSION IN SKELETAL MUSCLE OF RATS

作　　者：艾华[1] 陈吉棣[1] 贺师鹏[1] 

机构地区：[1]北京医科大学运动医学研究所,北京医科大学生物物理教研室

出　　处：《营养学报》1996年第1期76-80,共5页Acta Nutrimenta Sinica

基　　金：博士生研究经费

摘　　要：本文观察了缺锌对大鼠股四头肌深层肌总蛋白质、ＤＮＡ、总ＲＮＡ、肌动蛋白（ＤＮａｓｅⅠ抑制法）和α－肌动蛋白ｍＲＮＡ（地高辛标记探针，Ｎｏｒｔｈｅｒｎ杂交）含量的影响。结果显示：缺锌时肌肉中总蛋白质、ＤＮＡ和肌动蛋白的相对含量变化不明显，总ＲＮＡ和α－肌动蛋白ｍＲＮＡ的含量明显减少。这提示，锌缺乏导致α－肌动蛋白的基因表达下降，可能在转录和翻译过程中影响骨骼肌肌动蛋白的合成速率。he concentrations of zinc,total protein,DNA,total RNA,actin(by DnaseⅠassay),and α一actin mRNA(by dikroxigenin- labeled cDNA probe,Northern blot) were deterinined in the profouiid muscularis of m,quadriceps fenioris of niJe Sprague-Dawley rats with different zinc status: controlled rats fed with controlled diet(Zn 50mg/kg diet)for 3 weeks,zinc一deficient(ZnD) rats fed witli ZnD diet (Zn 1mg/kg diet)for 3 weeks,and zinc- reple-nished (ZnR) rats,wtih ZnD diet for 2 week5,and then controlled diet for 1 week.The results showed that the contents of zinc,total protein,DNA and actiiis iii the muscles of 3 groups of rats were not significantly different.The total RNA and α一actin mRNA decreased significantly in ZnD rats, and increasedagain inthe ZnR rats.It was suggested that the decr-eased a,actin gene expression by zinc deficiency may influence the synthe-tic speed of actin drt transcription and traiislation levels in skeletal inuscle.

关 键 词：锌缺乏 肌动蛋白 基因表达 骨骼肌 大鼠 

分 类 号：R591.1[医药卫生—内科学]