乙型肝炎血清标志物与HBV DNA的相关性分析  

The relationship between serum markers in hepatitis B patients and HBV DNA and clinical significance

作　　者：李志方[1] 吴晓蔓[1] 

机构地区：[1]广州医学院第二附属医院检验科,广东广州510260

出　　处：《中国热带医学》2006年第6期1027-1028,共2页China Tropical Medicine

摘　　要：目的探讨乙型肝炎患者血清标志物(HBV-M)与HBV-DNA的关系。方法采用酶联免疫吸附试验(ELISA)和荧光定量-PCR(FQ-PCR)法对1433例乙肝患者进行HBV-M以及HBV-DNA定量检测。结果在HBsAg(+)HBeAg(+)HBcAb(+)血清中HBV-DNA阳性率和含量最高,血清中HBeAg与HBV-DNA含量密切相关,但部分HBeAg阴性或HBeAb阳性患者也有较高的HBV-DNA阳性率及含量。结论单凭血清免疫标志物模式难以准确判断HBV的复制程度及传染性的强弱,定量检测HBV-DNA能真实反映HBV的复制情况,对诊断、治疗乙肝患者及疗效观察具有较大的指导意义。Objective To analyze the relationship between serum HBV - DNA levels and the patterns of serological markers. Methods The levels of HBV markers and HBV - DNA in the serum of 1433 hepatitis B patients were quantitatively determined by ELISA and FQ- PCR. Results There was a close relationship between serum HBV - DNA and HBeAg. The highest HBV- DNA levels were presented in sera positive both for HBsAg and HBeAg, higher HBV - DNA levels could also be observed in some sera with negative HBeAg and positive anti - HBe. Conclusion Quantitatively determination of the level of HBV - DNA can genuinely reflect the results of HBV replication and it is also of great significance for diagnosis and observation on the results in treatment of HBV patients.

关 键 词：乙型肝炎 FQ-PCR HBV-DNA HBV-M 

分 类 号：R512.62[医药卫生—内科学]