淋病奈瑟菌孔蛋白PIA真核表达系统的构建及序列分析  

Construction of An Eukaryotic Expression System pCI-PIA Carrying the Porin IA Gene from the Neisseria Gonorrhoeae and Sequence Determination

作　　者：贺超[1] 廖芳[1] 刘海鹏[1] 刘丽江[2] 

机构地区：[1]华中科技大学同济医学院病原生物学系,湖北武汉430030 [2]华中科技大学同济医学院同济医院妇产科

出　　处：《中国妇幼保健》2006年第11期1524-1525,共2页Maternal and Child Health Care of China

基　　金：湖北省卫生厅资助项目(No.JX1B037)

摘　　要：目的:构建淋病奈瑟菌孔蛋白PIA(Porin IA)基因的真核表达系统,为淋病DNA疫苗的研究提供材料。方法:PCR技术扩增淋病奈瑟菌捷克标准株的PIA基因片段,将其定向插入真核表达载体pC I-neo多克隆位点中,构建重组表达载体。并用酶切分析、PCR扩增及序列测定方法,对重组载体进行鉴定。结果:淋病奈瑟菌PIA基因被正确克隆到真核表达载体pC I-neo中,测序结果与Genebank中AF090819等菌株序列有99.99%同源性。结论:真核表达系统(pC I-PIA)构建成功。Objective： To construct an eukaryotic expression system carrying the Neisseria gonOrrhoeae Porin IA （PIA） gene. Methods： The PIA gene was amplified from the genome of the Neisseria gonorrhoeae by PCR and recombined with plasmid pCI -net. The recombinant plasmid pCI - PIA was verified with restriction analysis, PCR and sequence. Results： PIA gene was cloned correctly into vector pCI - net. Conclusion ： The eukaryotic expression system pCI - PIA was constructed successfully.

关 键 词：淋病奈瑟菌 PIA基因 真核表达 

分 类 号：R378[医药卫生—病原生物学]