耐力训练和注射DHEA对雄性大鼠肝脏、心脏和腓肠肌PPARα表达的影响  

Effects of Dehydroepiandrosterone and Endurance Training on PPARα Expression of Liver,Heart and Gastrocnemius Muscle in Male Rats

作　　者：陈敏[1] 方子龙[2] 张志文[3] 张林[1] 王启荣[2] 

机构地区：[1]苏州大学,苏州215021 [2]国家体育总局运动医学研究所,北京100029 [3]北京大学,北京100083

出　　处：《首都体育学院学报》2006年第3期33-37,共5页Journal of Capital University of Physical Education and Sports

基　　金：国家自然科学基金资助项目(30150009)-合成类固醇类激素运动性副作用及其危害的研究

摘　　要：本文探讨了持续性运动和/或注射脱氢表雄酮(DHEA)对雄性大鼠体重增长、血脂水平、以及肝脏、心脏及腓肠肌细胞内过氧化物体增殖活化受体α(PPARα)及其靶酶-酰基辅酶A氧化酶(AOX)的mRNA和PPARα蛋白量的影响。将40只SD大鼠随机分为4组:A为对照组,B为DHEA组,C为运动训练组,D为运动训练+DHEA组。DHEA给药方式:每天皮下注射20m g/kg的DHEA S,每周6d,共6周。运动方式:采用跑台,速度为25m/m in,每天1h,每周6d,共7周。结果发现:(1)D组实验后体重增长明显少于A组和B组(p<0.05)。(2)各组之间的总胆固醇、甘油三酯、高密度脂蛋白胆固醇和低密度脂蛋白胆固醇含量均无明显差异。(3)各组间的PPARα和AOX的mRNA表达均无明显差异。(4)在肝脏,C组和D组的PPARα蛋白水平明显高于A组(P<0.01和P<0.001)。在心脏,B组、C组和D组的PPARα蛋白水平明显高于A组(P<0.05、P<0.001和P<0.001)。在腓肠肌,D组的PPARα蛋白水平明显高于A组(P<0.05)。结果表明:(1)运动训练加注射DHEA可以明显控制体重的增长。这种作用可能不是通过降低血脂水平而实现的。(2)DHEA和持续性运动在翻译及翻译后水平上调了PPARα基因的表达。DHEA提高心脏内PPARα的水平,而持续性运动能诱导心脏和肝脏内PPARα的表达。DHEA结合运动训练诱导肝脏、心脏和腓肠肌的PPARα的表达,介导各脏器的脂肪分解。The purpose of this study is to discuss the effects of dehydroepiandrosterone （DHEA） and/or endurance training on the body weight,blood lipids ,the expression of peroxisome proliferator-activated receptor α （PPARα） and acyl-CoA oxidase （AOX） in some tissues in rats. Forty adult male Sprague-Dawley rats were randomly divided into four groups：group A （control） , group B （DHEA）,group C （training） and group D （DHEA and training）. DHEAS was administrated by subcutaneous injection at a daily dose of 20mg/kg body weight in group B and D for six weeks. Animals in group C and D received endurance training by running on the treadmill for one hour at a speed of 25m/ min,six days per week,for seven weeks. The results showed that： （1） The increased body weight in group D was less than in group A （P〈0. 05） and B （P〈0. 05） significantly. （2） There were no significant differences in serum concentration of total cholesterol, triglyceride, HDL C and LDL-C in all groups. （3） There were no significant differences in mRNA expression levels of PPARα and its target gene acyl-CoA oxidase （AOX） in liver,heart and gastrocnemius muscle in all groups. （4） The level of PPARα protein was significantly higher in group C （P〈0. 01） and D （P〈 0. 001） than that in the control group in liver. The level of PPARα protein was significantly higher in group B （P〈0. 05） ,C （P〈0. 001） and D （P〈0. 001） than in the control group in heart. The level of PPARα protein was significantly higher in group D （P〈0.05） than in the control group in gastrocnemius muscle. The results also indicated that： （1） DHEA combined with endurance training could reduce the weight gain. This effect might not be related to the blood lipids level. （2） DHEA and endurance training up-regulated the expression of PPARα gene in translation and post-translation level. DHEA led to the higher level of PPARα protein in heart,endurance training led to the higher level of PPARα

关 键 词：过氧化物体增殖活化受体α 脱氢表雄酮 训练 酰基辅酶A氧化酶 血脂 大鼠 

分 类 号：G804.7[文化科学—运动人体科学]