糖尿病及高危人群血清游离脂肪酸成分分析  

Analysis on serum free fatty acid composition in patients with type 2 diabetes mellitus

作　　者：李嘉强[1] 戴颖秀 刘玉敏[2] 郑基 刘文斌 

机构地区：[1]上海市杨浦区疾病预防控制中心,200090 [2]上海交通大学分析测试中心 [3]上海市杨浦区平凉社区卫生服务中心 [4]上海市杨浦区五角场社区卫生服务中心

出　　处：《中国公共卫生》2006年第6期690-692,共3页Chinese Journal of Public Health

基　　金：上海市杨浦区卫生局科研基金资助(2002AA001-005)

摘　　要：目的研究2型糖尿病及高危人群血游离脂肪酸成分水平及其与血糖参数间关系.方法用气相色谱/质谱（GC/MS）联用技术测定162例2型糖尿病及高危人群与43名正常对照组血游离脂肪酸成分水平.结果糖尿病组不饱和脂肪酸（C18：2,C18：1,C20：4,C22：6,C20：3）高于正常对照组,在C18：2,C20：3的差异有统计学意义（P＜0.01～P＜0.05）;不饱和脂肪酸/总脂肪酸（UFA/TFA）、多不饱和脂肪酸/总脂肪酸（PUFA/TFA）、多不饱和脂肪酸/饱和脂肪酸（PUFA/SFA）显著高于正常对照（P＜0.01）;饱和脂肪酸（C16：0）和SFA/TFA低于正常对照组,差异有统计学意义（P＜0.01）;亚油酸（C18：2）、n6/n3显著高于高危人群组（P＜0.05～P＜0.01）.高危人群组UFA/TFA、PUFA/SFA显著高于正常对照组（P＜0.05）.在研究对象中,血糖与UFA/TFA、PUFA/TFA、PUFA/SFA、n6/n3呈正相关（r=0.190,0.172,0.190,0.182,P＜0.01～P＜0.05）,血糖与SFA/TFA呈负相关（r=-0.190,P＜0.01）.结论2型糖尿病及高危人群中存在游离脂肪酸代谢紊乱.n6系多不饱和脂肪酸升高,可能在糖尿病发病中起重要作用.调整膳食结构,可以减少脂质过氧化损伤.Objective To explore the relationship between serum free fatty acid（FFA） composition and fasting blood glucose （FBG） in patients with type 2 diabetes mellitus and risk factors associated with diabetes（RFD）. Methods The serum FFA profile was measured with the gas chromatography and mass spectrometer（C-C/MS）in 162 patients and 43 control subjects. Results The patients with diabetes had higher parameters of unsaturated fatty acid （ UFA, C18 ： 2, C18 ： 1,C20 ： 4, C22 ： 6, C20 ： 3 ）, and polyunsaturated fatty acid （ PUFA, C18 ： 2, （320 ： 3 ）, UFA/TFA, PUFA/TFA and PUFA/SFA showed a significant increase as compared with those in the normal subjects （P〈0.01-P〈0.05 ）. Saturated fatty acid （SFA, C16：0） and SFA/TFA in patients with diabetes were decreased remarkably than that in the healthy controls（P〈0.01 ）, their linoleic acid （C18：2） and n6/n3 were significantly increased compared to the patients with RFD（ P 〈 0.05 - P〈 0.01 ）. Subjects with RFD had higher ratio of UFA/TFA, PUFA/SFA compared to the healthy controls（ P 〈 0.05 ）. FBG had remarkably positive correlation with UFA/TFA, PUFA/TFA, PUFA/SFA and n6/n3 （ r=0.190,0.172, 0.190,0.182, P〈0.01 - P〈0.05, respectively） and a negative correlation with SFA/TFA（ r = - 0.190, P〈0.01 ） in these all subjects. Conclusion There is the FFA metabolic abnormality in these patients. Increased levels of n6PUFA may play a role in diabetic pathogenesis. Balanced dietary may improve upon the increased lipid peroxidation.

关 键 词：游离脂肪酸 2型糖尿病 气相色谱/质谱联用技术 

分 类 号：R587.1[医药卫生—内分泌]