亚急性甲醛吸入对小鼠肝细胞DNA损伤作用  

Damage effect of subacute formaldehyde on hepatocytic DNA in mice

作　　者：王丽[1] 乔琰[1] 鲁志松[1] 丁书茂[1] 严彦[1] 杨旭[1] 

机构地区：[1]华中师范大学生命科学学院环境科学实验室,武汉430079

出　　处：《中国公共卫生》2006年第6期709-710,共2页Chinese Journal of Public Health

基　　金：国家科技部"十五"科技攻关计划基金资助(2001BA704B01;2004BA809B0604;2004BA809B0605)

摘　　要：目的检测亚急性甲醛吸入对小鼠肝细胞的损伤作用,并探讨用归一化处理方法解决单细胞凝胶电泳重复性差的可行性。方法将小鼠分别暴露于不同浓度的气态甲醛(0.5,1.0和3.0 mg/m3)中染毒14 d,每天6 h,用单细胞凝胶电泳技术检测肝细胞DNA的损伤程度。结果0.5和1.0 mg/m3的甲醛可造成DNA的严重断裂(P<0.001),而3.0 mg/m3的甲醛则引起显著的交联作用,归一化的结果与之相符,并表明不同浓度的甲醛导致不同程度和类型的DNA损伤。结论甲醛可造成机体内肝细胞DNA的断裂和交联,该损伤可能是甲醛致癌机制之一;归一化可以弥补单细胞凝胶电泳实验重复性不佳的缺陷。Objective To explore the hepatocellular DNA damage after subacute formaldehyde exposure and the application of normalization as a statistic method to improve the reproducibility of single cell gel electrophoresis（ SCGE, comet assay）. Methods Mice were respectively exposed to different concentrations of gaseous formaldehyde for 14 d, 6 h per day.SCGE was performed to detect the DNA damages. And normalization was used to discuss the reproducibility of SCGE assay.Results Formaldehyde could induce different kinds of DNA damages： formaldehyde caused significant DNA strands breaks at concentrations of 0.5 and 1.0 mg/m^3 （ P〈0.001 ） while crosslinks were induced at 3.0 mg/m^3. Results from normalization were in consistent with the former results and indicated that DNA damages were enhanced with the increase of formaldehyde level. Conclusion Formaldehyde was both a DNA strands breaks and crosslinks agent to the hepatocytes in vivo, which may be one of the possible mechanisms of formaldehyde as a carcinogen. Normalization could improve the reproducibility of SCGE assay.

关 键 词：气态甲醛 肝细胞DNA损伤 单细胞凝胶电泳 归一化 

分 类 号：R122.1[医药卫生—环境卫生学]