PCR技术在赫坎按蚊复合体近缘种鉴别中的应用  

Application of Polymerase Chain Reaction-restriction Fragment Length Polymorphism Techniques in Differential Identification of Close-breeding Anopheline Mosquitoes from Anopheles hyrcanus Complex

作　　者：陈国英[1] 黄光全[1] 周华云[2] 张华勋[1] 李书华[1] 王伟民[2] 曹俊[2] 袁方玉[1] 

机构地区：[1]湖北省疾病预防控制中心传染病研究所,武汉430079 [2]江苏省寄生虫病防治研究所

出　　处：《中国媒介生物学及控制杂志》2006年第3期170-172,共3页Chinese Journal of Vector Biology and Control

基　　金：国家"十五"科技攻关课题(2001BA705B09)

摘　　要：目的应用聚合酶链反应(PCR)技术分析湖北省不同地区媒介按蚊的种型。方法采用传统的形态学方法和新建立的基因鉴别技术对现场捕获的按蚊分别进行形态特征鉴别和基因鉴别及比较。结果现场捕获181只按蚊,形态学确认176只为中华按蚊,而PCR鉴定172只为中华按蚊,另4只为八代按蚊;经形态学特征鉴别的5只嗜人按蚊中,PCR鉴别有4只为嗜人按蚊,另1只为八代按蚊。结论采用PCR基因鉴别技术能准确鉴别赫坎按蚊种团内中华按蚊、嗜人按蚊、八代按蚊等近缘种按蚊,较传统的按蚊形态学鉴别方法准确,适用于复合媒介地区的疟疾媒介调查和监测。Objective To identify species of anopheles vectors captured in varied areas of Hubei province with polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） techniques. Methods Anopheline mosquitoes （ Anopheles hyrcanus complex） captured at the fields of Hubei province were identified with traditional morphological classification and newly-established genetic methods （PCR-RFLP） based on the sequences of their ribosomal DNA in the ITS2 region. DNA sequence in Anopheles anthropophagus, Anopheles sinensis and Anopheles yatsushiroensisi were analyzed and compared. Results A total of 181 anopheline mosquitoes were captured and identified, including 176 identified as Anopheles sinensis and five as Anopheles anthropophagus according to their morphological features, and 172 identified as Anopheles sinensis, four as Anopheles anthropophagus and one as Anopheles yatsushiroensisi by PCR-RFLP. Conclusion Close-breeding anopheles, such as Anopheles sinensis, Anopheles yatsushiroensisi and Anopheles anthropophagus in Anopheles hyrcanus complex could be accurately identified by PCR-RFLP, which was suitable for the investigation and surveillance for malariavector mosquitoes in the areas with anopheles complex.

关 键 词：嗜人按蚊 中华按蚊 八代按蚊 核糖体核酸内转录间隔2 聚合酶链反应 

分 类 号：R384.1[医药卫生—医学寄生虫学]