用多引物聚合酶链反应检测鼠疫耶尔森菌  

Detection of Yersina pestis by Polymerase Chain Reaction with Multiplex Primers

作　　者：吴明寿[1] 夏连续[2] 张志凯[2] 钟佑宏[1] 郭英[1] 海荣[2] 俞东征[2] 董兴齐[1] 

机构地区：[1]云南省地方病防治所中心实验室,大理671000 [2]中国疾病预防控制中心传染病预防控制所

出　　处：《中国媒介生物学及控制杂志》2006年第3期221-223,共3页Chinese Journal of Vector Biology and Control

摘　　要：目的建立多引物检测鼠疫耶尔森菌(鼠疫菌)的PCR(M-PCR)方法。方法合成4对引物,分别来源于质粒和染色体DNA上F1、pla、HmsI、nv基因,对164株鼠疫菌进行扩增。结果在164株鼠疫菌中,有152株菌4种基因扩增均阳性,仅云南省12株Hms基因扩增为阴性。结论采用M-PCR方法检测鼠疫菌DNA具有较好的敏感性、特异性和稳定性,其可作为检测与鉴别鼠疫菌和疫情监测方面快速诊断的方法。Objective To develop a polymerase chain reaction （PCR） method to detect Yersina pestis by multiplex primers （M-PCR）. Methods Four pairs of primers, originated from the genes F1 （specific capsular antigen fraction 1）, pla （palsrninogen activator）, Hrns and lnv encoded on the two kinds of 65×10^6 plasmids and two chromosomal DNA, were designed and 164 strains of Y. pestis were amplified with multiplex primers. Results One hundred and fifty-two of 164 strains of Y. 1）estis showed positive in M-PCR, Only 12 strains of them isolated from Yunnan were negative with amplification of the Hms gene. Conclusion M-PCR method showed satisfactory sensitivity, specificity and stability for detecting and identifying Y. pestis DNA and could be used in surveillance and ranid diagnosis for plague.

关 键 词：鼠疫耶尔森菌 毒力基因 多引物 聚合酶链反应 

分 类 号：R516.8[医药卫生—内科学]