薄层扫描法测定血清中胺碘酮的浓度  

Determination of amiodarone in human serum by TLC

作　　者：王锦秋[1] 王民[2] 丰艳梅[1] 王春梅[1] 

机构地区：[1]西安交通大学第二医院药剂科,陕西西安710004 [2]西安交通大学产业集团,陕西西安710061

出　　处：《华西药学杂志》2006年第3期300-302,共3页West China Journal of Pharmaceutical Sciences

摘　　要：目的建立血清中胺碘酮的测定方法。方法用TLC法，以含羧甲基纤维素钠的硅胶60GF254为固定相，无水乙醇-氨水（50：1）为展开剂，检测波长λs=242nm。结果胺碘酮的点样量在0．5—6．0μg范围内与峰面积的线性关系良好（r=0．9969），平均回收率为95．79％±3．04％。结论所建方法操作简便、灵敏、准确，适用于胺碘酮的常规血药浓度监测。OBJECTIVE To establish a method for determination of Amiodarone in serum.METHODS A TLC method was used. The stationary phase is silica GF254 containing CMC - Na. Absolute ethanol- ammonia liquor （50： 1） was used as the developing solvent.The detective wavelength was 242 nm. RESULTS Amiodarone at the concentration of 0.5 - 6.0 μg showed a good linearity（ r = 0.9969） .The average recovery was 95.79% with RSD of 3.04%. CONCLUSION The method appeares to be simple, sensitive and accurate. It can be used in therapeutical drug monitoring of Amiodarone.

关 键 词：胺碘酮 薄层色谱法 血药浓度监测 血清 

分 类 号：R917[医药卫生—药物分析学]