富锗绞股蓝组织培养的研究  

STUDIES ON ACCUMULATING Ge OF TISSUE CULTURE GYNOSTEMMA PENTAPHYLLUM

作　　者：王习霞[1] 屠春燕[1] 萧民耕 欧阳平凯[1] 韦萍[1] 

机构地区：[1]南京化工大学生物工程与科学系,南京210009

出　　处：《南京化工大学学报》1996年第1期13-16,共4页Journal of Nanjing University of Chemical Technology(Natural Science Edition)

摘　　要：将绞股蓝愈伤组织细胞接种在加有不同浓度锗的培养基中，在黑暗条件下进行组织培养，结果发现：通过植物细胞吸收转化可将无机锗化为有机锗，并富集在绞股蓝细胞内，提高了绞股蓝的药用价值。研究观察了培养基中加入锗对绞股蓝愈伤组织细胞中的总皂甙、氨基酸的含量以及细胞生长量的影响。The gynosstemma pentaphyllum cells were grown on media containing different types of Ge under dark condition culture. The results show that gynostemma pentaphyllum cells can absorb Ge, and turn inorganic Ge to organin Ge. Simultaneously, Ge was accumulated into gynostemma pentaphyllum. The effects of media with added Ge on contents of saposide, amino acid in gynostemma Pentaphyllum cells and cell biomass were also studied.

关 键 词：绞股蓝 组织培养 锗 愈伤组织 培养基 

分 类 号：S567.239[农业科学—中草药栽培]