高羊茅愈伤组织诱导及植株再生的研究  

rom established embryogenic cell suspension cultures of Italian ryegrass and tall fescue\.Plant Breeding,1990,104:265-271.Callus induction and plantlet regeneration of Tall Fescue

作　　者：王维飞[1] 韩烈保[1] 曾会明[1] 

机构地区：[1]北京林业大学草坪研究所,北京100083

出　　处：《草业科学》2006年第6期99-103,共5页Pratacultural Science

基　　金：国家转基因植物研究与产业化专项项目(J2002-B-006);国家"863"项目(2001AA244082);新世纪优秀人才支持计划

摘　　要：对高羊茅Festuca arundinacea4个品种的成熟种子进行了愈伤组织诱导及植株再生的研究,建立了高羊茅的组织培养体系。结果表明:1)外植体种子的消毒程序以“次氯酸钠(活性氯≥5%)1 h+无菌水搅拌去皮”最佳;2)4个高羊茅品种以“千年盛世”在愈伤诱导中优势最突出;3)“千年盛世”愈伤诱导的适宜培养基为:MS+9 mg/L 2,4-D+300 mg/L水解酪蛋白;4)愈伤组织再生培养基中6-BA的适宜浓度为0.05 mg/L,KT的适宜浓度为0.2 mg/L。In order to establish the tissue culture system of tall rescue （Festuca arundinacea ） for the transgenic study, callus induction and plantlet regeneration of sterilized mature seeds of tall rescue varieties were studied. The results indicated that： OThe best surface sterilization process for seeds was stirred in NaClO（active chlorin≥5%） one hour and then in sterilized water for dissolving the skin of seeds.②Millennium performed better than other lines of tall rescue in callus induction. ③The suitable medium for callus induction of Millennium was a basic MS salt mixed with 9 mg/L 2,4-D＋300 mg/L Hydrolysate. ④The suitable concentration of 6-BA and KT in medium for plantlet regeneration were 0.05 mg/L and 0. 2 mg/L.

关 键 词：高羊茅 愈伤组织 植株再生 培养基 2 4-D 6-BA KT 

分 类 号：S543.9[农业科学—作物学]