新型佐剂在破伤风抗毒素血浆生产中的应用研究  

Study on new types of adjuvant used for production of tetanus antitoxin plasma

作　　者：陈孝婷[1] 谢小荣[1] 申联滨[1] 彭恭嘉[1] 

机构地区：[1]兰州生物制品研究所,兰州730046

出　　处：《微生物学免疫学进展》2006年第2期35-38,共4页Progress In Microbiology and Immunology

摘　　要：应用两种新型佐剂(Montan inde ISA 50V和Montan inde ISA 206)分别乳化精制破伤风类毒素制备成油乳抗原,免疫豚鼠三程,并与弗氏不完全佐剂(FIA)进行对比;在弗氏不完全佐剂免疫马匹到第7程后,改换新型佐剂抗原免疫三程(第8、9、10程)。采用小鼠中和试验测定血清效价。结果表明,两种新型佐剂使用安全,稳定性良好,且操作方便;使用Montan inde ISA 50V免疫豚鼠与马匹的免疫效果均有明显优势,Montan inde ISA 206次之,FIA效果相对较差。表明新型佐剂Montan inde ISA 50V的应用前景良好。Two new types of adjuvant,Montaninde ISA 50V and Montaninde ISA 206,were separately emulsified with purified tetanus toxoid in preparation of emulsion antigens which were used to immunize guinea pigs and horses,and compared the obtained effects with those by using Fruend's incomplete adjuvant(FIA).Serum titers were tested by mouse neutralization test.The results showed that the effect of Montaninde ISA 50V was the best,Montaninde ISA 206 second.Two new types of adjuvant were safe,easily performed and with good stabilities.It indicates that Montaninde ISA 50V has a prospective application in immunization procedure.

关 键 词：新型佐剂 精制破伤风类毒素 免疫 效价 

分 类 号：R392[医药卫生—免疫学]