TUNEL法检测乳酸杆菌对小鼠宫颈鳞癌的凋亡诱导作用  

Study on the apoptosis induced by Lactobacillus on U_(14) by TUNEL

作　　者：李秀真[1] 曹蕊[2] 曹卉[1] 陈廷[1] 刘昌平[1] 山长武[1] 

机构地区：[1]济宁医学院微生物学教研室,山东济宁272013 [2]济宁医学院附属医院

出　　处：《中国微生态学杂志》2006年第3期189-190,193,共3页Chinese Journal of Microecology

基　　金：济宁市科技局课题(2002)

摘　　要：目的应用脱氧核苷酸转移酶介导的dUTP缺口末端标记法———TUNEL法,检测乳酸杆菌(Lactoba-cillus,LB)对小鼠宫颈鳞癌的凋亡诱导作用。方法建立615近交系小鼠U14移植瘤动物模型,并随机分组,TUNEL法检测各组细胞凋亡指数(apoptosis index,AI)。结果乳酸杆菌实验组的AI为(11.25±4.06)%,与对照组的差异有非常显著性(P<0.01),与抗癌药顺铂组的差异有显著性(P<0.05)。结论乳酸杆菌可诱导U14移植瘤细胞凋亡,这可能是其抑癌机制之一。Objective To detect the apoptosis induced by Lactobacillus （LB） on murine cervical carcinoma U14 by TUNEL. Methods The 615-strain mice with U14 were randomly divided into three groups. The apoptosis index（AI） was assessed by TUNEL. Results AI of LB group was （ 11.25 ± 4.06） %. Marked difference was found in AI between LB and DDP, LB and control, DDP and control. Conclusion Lactobacillus could induce apoptosis of routine cervical cancer. It was a possible way of antitumor mechanism of Lactobacillus.

关 键 词：TUNEL法 乳酸杆菌 小鼠宫颈鳞癌14号 细胞凋亡 

分 类 号：R378.992[医药卫生—病原生物学]