昆明婴幼儿腹泻的轮状病毒分子基因特征研究  

Rotavirus molecular genic feature of infants with diarrhea in Kunming

作　　者：黄永坤[1] 甄双平[1] 侯宗柳[2] 戚勤[1] 庞伟[2] 

机构地区：[1]昆明医学院第一附属医院儿科,云南昆明650032 [2]中国医学科学院医学生物研究所,云南昆明650118

出　　处：《中国微生态学杂志》2006年第3期199-200,共2页Chinese Journal of Microecology

摘　　要：目的了解昆明婴幼儿腹泻中轮状病毒感染情况。方法收集昆明医学院第一附属医院儿科2004年10-12月住院的腹泻的患儿粪便标本60份，采用聚丙烯酰胺凝胶电泳（PAGE）法和RT—PCR法进行轮状病毒分型。结果60份粪便标本中，29份检测到A组轮状病毒RNA基因，阳性检出率为48.3％，未发现B组及C组轮状病毒。其中RNA长型有29份（100％），未发现短型和混合型。对29份有明确编号的轮状病毒阳性标本来源的患儿分析显示，平均发病年龄10.5个月。29份标本中，有20份可用RT—PCR分型，均为G3型，未发现其他型。结论A组轮状病毒是昆明地区5岁以下婴幼儿腹泻病的主要病原，3月龄-2岁婴幼儿是轮状病毒的易感人群，以冬季10月份至12月份为流行高峰，基因组以长型为主，血清型为G3型。Objective To investigate rotavirus infection of infants with diarrhea in Kunming,China. Methods Stool samples were collected from 60 children who were hospitalized in the department of pediatric of the First Affiliated Hospital of Kunming Medical college from October to December in 2004. Polyacrylamide gel electerphoresis （PAGE） was used to detect rotavirus genic RNA and indentify electropherotypes of group A rotavirus. Reverse transcription polymerase chain reaction （RT-PCR） was performed to indentify rotavirus G serotypes. Results Out of 60 samples investigated, 29 （48.3%） were group A rotavirus gene positive by PAGE, and these showed long electrophrotypes, none showed short electrophrotypes or mixed electrophrotypes. Mean age was 10. 5 months. RT-PCR were performed for 29 fecal specimens and VP7 gene products were obtained from 29 （ 100% ） samples. Nested-PCR showed serotypc G3 accouted for 100 （29）. Conclusions Group A rotavirus was the major pathogen of diarrhea in infants below 5 years old in Kunming. The children with 3 months old to 2 years old were susceptible to rotavirus and the summit infection were in winter from October to December, Gcnic was long electorpherotypes and serotype was G3.

关 键 词：轮状病毒 腹泻 婴幼儿 分子基因 

分 类 号：R723.11[医药卫生—儿科]