两种动物血巧克力平板分离流感嗜血杆菌效果评价  

Effect evaluation of chocolate medium made of two kinds of animal blood using for isolate Haemophilus influenzae

作　　者：黄旭强[1] 邓力[1] 邓秋连[1] 谢永强[1] 李梅爱[1] 陈卓瑶[1] 

机构地区：[1]广州市儿童医院,广东广州510120

出　　处：《中国微生态学杂志》2006年第3期255-256,共2页Chinese Journal of Microecology

基　　金：广东省科技计划项目(2005B36001022)

摘　　要：目的比较羊血、马血巧克力平板痰培养流感嗜血杆菌的检出率和生长情况。方法将送检的痰标本及流感嗜血杆菌标准菌株ATCC10211分别接种于羊血、马血巧克力平板上，置烛缸中，36气培养24h，比较流感嗜血杆菌检出率及其生长情况。结果（1）2种巧克力平板痰培养流感嗜血杆菌的检出率分别为8％、34．5％。（2）标准菌株ATCC10211在马血巧克力平板上生长良好，菌落直径0．5-1．0mm，而在羊血巧克力平板上生长差，菌落大小如“沙粒”，肉眼难见。结论痰培养流感嗜血杆菌使用马血巧克力平板效果优于羊血巧克力平板。Objectives To compare the isolate rates of Haemophilus influenzae in sputum by chocolate plates made of sheep and horse blood, and the growth of Haemophilus influenzae on the two kinds of chocolate plates. Methods Sputums and Haemophilus influenzae ATCC 10211 were streaked on the two kinds of chocolate plates and incubated at 36 ℃ in a candle jar for 24 hours. The isolate rates of Haemophilus influenzae by two kinds of chocolate plates and the growth of Haemophilus influenzae ATCC 10211 were compared. Results The isolate rates of Haemophilus influenzae in sputum by chocolate plates made of sheep blood, horse blood were 8% ,34.5% respectively. Haemophilus influenzae ATCC 10211 grew very well on chocolate plates made of horse blood,its diameter of colony was 0.5 - 1.0 mm. But it did not grow well on the chocolate plates made of sheep blood, its colony was as small as a sand and hard to be seen with naked eye. Conclusions Chocolate plates made of horse blood can be used to isolate Haemophilus influenzae in sputum was better than the chocolate plate made of sheep blood.

关 键 词：动物血 巧克力平板 流感嗜血杆菌 检出率 

分 类 号：R378.41[医药卫生—病原生物学]