^(99)Tc^m-环丙沙星一步法药盒的制备及其动物体内分布  

Preparation of ^(99)Tc^m-ciprofloxacin Kit and Its Biodistribution

作　　者：余媛[1] 朱霖[1] 蒋宁一[2] 汪建军[1] 张弘[2] 

机构地区：[1]北京师范大学化学系放射性药物教育部重点实验室,北京100875 [2]中山大学第二附属医院核医学科,广东广州510120

出　　处：《同位素》2006年第2期92-95,99,共5页Journal of Isotopes

摘　　要：确定了99Tcm-环丙沙星(ciprofloxacin,CPF)的最佳标记条件,在此基础上制备了环丙沙星冻干品药盒,并初步观察了99Tcm-CPF在炎症模型小鼠体内的分布。结果显示最佳标记条件为:盐酸环丙沙星的用量大于4 mg;SnCl2.2H2O的用量大于100μg,体系pH为3.0~3.5,反应时间约10 min。最佳标记条件下制备的99Tcm-CPF放化纯度>95%,室温放置6 h,其放化纯度无明显变化;药盒分别在0~8℃冷藏及-18℃冷冻保存3个月后再标记,标记物的放化纯度仍>95%;小鼠炎症模型实验结果显示,注射后4 h脓肿与肌肉放射性摄取比为4.30±0.31。Ciprofloxacin （CPF） made in China is labeled with ^99Tc^m and the factors which affects the labeling efficiency are determined. Accordingly, a new CPF cold kit is developed and the biodistribution of ^99Tc^m-CPF in infected animal is evaluated. Under the optimal conditions of pH 3.0-3.5, CPF contents above 4 mg, SnCl2 · 2H2O contents over 100μg, 10 minutes after injection of ^99Tc^mO4^-, the radiochemical purity of ^99Tc^m-CPF is above 95% and remained ）〉95% at 6 h after preparation at room temperature. The kit is stored at0- 8℃ or -18 ℃ in refrigerator, the radiochemical purity of ^99Tc^m-CPF keeps up with over 95 % after 3 months. Mice with infectious lesions injected with ^99 Tc^m-CPF show a mean abscess-to-muscle ratio of 4.30±0.31 at 4 h post injection.

关 键 词：^99TC^M 环丙沙星 炎症 药盒 

分 类 号：TL923[核科学技术—核燃料循环与材料] R817-33[医药卫生—影像医学与核医学]