基于聚硫堇和纳米金固定辣根过氧化物酶的生物传感器  

A Biosensor Based on Immobilizing Horseradish Peroxidase on Poly(Thionine) and Au Colloid as Matrixes

作　　者：石银涛[1] 袁若[2] 王娜[1] 柴雅琴[1] 张凌燕[1] 贺秀兰[1] 

机构地区：[1]西南大学化学化工学院,重庆400715 [2]重庆市现代分析化学重点实验室,重庆400715

出　　处：《西南师范大学学报（自然科学版）》2006年第3期80-84,共5页Journal of Southwest China Normal University(Natural Science Edition)

基　　金：国家自然科学基金资助项目(29705001);重庆市自然科学基金资助项目(CSTC2005BB4100);西南大学博士基金资助项目(SWNUB2004021)

摘　　要：用电化学聚合法在铂丝电极上制备聚硫堇，将其作为电子媒介体并且用于化学吸附纳米金，然后通过纳米金来固定辣根过氧化物酶，最后用聚乙烯缩丁醛包埋修饰好的电极，从而制得了新型过氧化氢生物传感器．该传感器还原峰峰电流与H2O2的浓度在2．15×10^-4～1.43×10^-2mol／L范围内呈良好的线性关系，检出限为2．00×10^-7mol／L，相关系数为0．998．实验结果表明，此方法具有较好的灵敏度和抗干扰能力，同时具有良好的稳定性和重现性。An amperometric biosensor for the determination of H2 O2 has been developed by means of immobilizing horseradish peroxidase onto a platinum electrode based on Au colloids and poly（thionine）as matrixes. Thionine, as a mediator, was first polymerized on a platinum electrode surface, then gold nanoparticles were chemisorbed onto ploy（thionine）, furthermore, horseradish peroxidase（HRP） was absorbed on the electrode surface through the gold nanoparticles conjugation. Finally, HRP and Au colloids were firmly immobilized on the electrode surface by the entrapping of polyvinyl butyral（PVB）. The performance and factors influencing the performance of the biosensor were studied. The biosensor exhibited a dynamic range of 2.15×10^-6-1.43×10^- 2 mol/L to H2O2 with a detection limit of 2.00×10^-7 mol/L and a correlation coefficient of 0. 998. Moreover, the biosensor exhibited high sensitivity, good repeatability and long-time stability.

关 键 词：聚硫堇 辣根过氧化物酶 纳米金 电聚合 

分 类 号：O657.15[理学—分析化学]