一氧化碳和一氧化氮对热性惊厥大鼠海马神经元凋亡的影响  

Effect of carbon monoxide and nitric oxide on the apoptosis of hippocampal neurons in rats with febrile seizures

作　　者：杨志仙[1] 秦炯[1] 常杏芝[1] 韩颖[1] 单英 

机构地区：[1]北京大学第一医院儿科,北京100034

出　　处：《中国当代儿科杂志》2006年第3期231-234,共4页Chinese Journal of Contemporary Pediatrics

基　　金：卫生部临床学科重点项目基金(编号20010912)

摘　　要：目的热性惊厥(FS)是小儿时期最常见的惊厥性疾病,反复FS可造成海马神经元的损伤,同时血红素氧合酶(HO)/一氧化碳(CO)系统和一氧化氮合酶(NOS)/一氧化氮(NO)系统明显上调并相互影响。该研究使用HO的抑制剂锌原卟啉Ⅸ(ZnPP-Ⅸ)和NOS的抑制剂Nω硝基-L-精氨酸甲酯(L-NAME)对FS时两个系统进行干预,探讨此两个系统对反复FS大鼠海马神经元凋亡的影响。方法采用热水浴诱导大鼠FS,隔日诱导1次,共诱导10次。21日龄SD大鼠随机分为4组对照组,FS组,FS+ZnPP-Ⅸ组,FS+L-NAME组。TUNEL法对各组大鼠海马CA1区神经元进行凋亡检测。结果FS组大鼠海马CA1区凋亡细胞数较对照组多225%,两组比较,差异有显著性(P<0.01),FS+ZnPP-Ⅸ组大鼠海马CA1区凋亡细胞数较FS组和对照组分别多62%和425%(均P<0.01),FS+L-NAME组大鼠海马CA1区凋亡细胞数较FS组低38%(P<0.01),较对照组多100%(P<0.05)。结论反复FS时,外源性给予HO抑制剂ZnPP-Ⅸ可导致海马神经元的凋亡增多,而NOS的抑制剂L-NAME可致海马神经元的凋亡减少,提示HO/CO系统可保护FS神经元的损伤,NOS/NO系统可加重FS神经元的损伤。Objective Febrile seizure （FS） is the most common type of seizure disorders in children. Recurrent FS can cause hippocampal neurons injury. At the same time heine oxygenase / carbon monoxide （HO/CO） system and nitric oxide sythase / nitric oxide （NOS/NO） system were up-regulated and interacted each other. This study examined the effects of the two systems on the apoptosis of hippocampal neurons in rats with recurrent FS. Methods FS was induced in rats by exposure to warm water bath （45.2 ℃ ）, once every 2 days, 10 times in all. Sprague-Dawley （SD） rats aged 21 days were randomly assigned into four groups： Control （37 ℃ water bath exposure）, FS, FS ＋ ZnPP-Ⅸ （HO inhibitor） and FS ＋ L-NAME （ NOS inhibitor） groups. The apoptosis of hippocampal CA1 neurons was detected by TUNEL. Results After recurrent FS, the apoptotic cells in the hippocampal CAI neurons increased by 225% compared with those in the Control group （P 〈0.01 ）. The apoptotic cells in the FS ＋ ZnPP-IX group increased by 62% and 425% compared with those in the FS and the Control groups （ both P 〈 0.01 ）. The apoptotic cells in the FS ＋ L-NAME group decreased by 38% compared with those in the FS group （P 〈0.01 ） and increased by 100% compared with those in the Control group （ P 〈0.05）. Conclusions In recurrent FS, exogenous administration of HO inhibitor ZnPP-Ⅸ may induce an increase of apoptotic cells in hippocampal neurons, while NOS inhibitor L-NAME may decrease the apoptotic cells. The results suggest that the HO/CO system might alleviate neuronal damage, while NOS/NO system might augment neuronal damage.

关 键 词：惊厥 发热性 一氧化碳 一氧化氮 凋亡 大鼠 

分 类 号：R720.597[医药卫生—急诊医学]