血管紧张素Ⅱ对大鼠胰岛β细胞形态功能的影响  

Effects of angiotensin II on morphology and function of islets β-cells in rats

作　　者：刘萍[1] 何岚[1] 王毅[1] 李建宁[1] 叶枫[1] 刘治全[2] 

机构地区：[1]西安交通大学第一医院内分泌科,陕西西安710061 [2]西安交通大学第一医院心内科,陕西西安710061

出　　处：《第四军医大学学报》2006年第11期968-970,共3页Journal of the Fourth Military Medical University

摘　　要：目的:探讨缓慢输注血管紧张素Ⅱ(AngⅡ)后,大鼠胰岛β细胞功能和形态的变化.方法:给予SD大鼠皮下缓慢输注AngⅡ2wk,成模后再分别给予正常盐(8g/L)和高盐(40g/L)饮食,动态观察血压、血糖、血胰岛素变化,并利用免疫组化的方法了解胰岛β细胞形态的变化.结果:AngⅡ输注后2wk,大鼠血压明显升高;停止AngⅡ输注后4wk,实验组血压继续升高,实验+高盐组血压升高更明显,实验+高盐组β细胞面积明显大于对照组(P<0.05);停止AngⅡ输注后12wk,实验组血压比对照组血压仍高,血胰岛素均明显降低,胰岛β细胞面积明显增大(P<0.05),实验+高盐组变化更明显.结论:缓慢输注AngⅡ可引起血压明显升高,可能影响胰岛β细胞结构,导致β细胞胰岛素分泌功能受损.AIM： To study the effects of angiotensin Ⅱ （Ang Ⅱ ） on the function and morphology of islets β-cells. METHODS： The SD rats accepted Ang Ⅱ by hypodermic injection with the osmotic minipump for 2 weeks. Hereafter, they were fed with a normal （8 g/L NaCl） or high-salt （40 g/L NaCl） diet respectively. The following indexes, including blood pressure, fasting blood glucose, insulin and the morphology of islets β-cells were observed. RESULTS： The blood pressure of rats was significandy elevated 2 weeks after accepting Ang Ⅱ via chronic hypodermic injection; at the end of 4 weeks, compared with control group, the blood pressure was increased unceasingly and markedly in the rat with Ang Ⅱ infusions; meanwhile, the areas of islets β-cells in the rats of high-salt diet became larger than those of control group （P〈0.05）; at the end of the 12 weeks, the blood pressure of rats with Ang Ⅱ infusions was higher than that of control group, in addition, except the fasting blood glucose, the fasting insulin was significantly decreased and the areas of islets β-cells significantly increased （ P 〈 0.05 ）, and high-salt loading exacerbated the effects of Ang Ⅱ infusion significantly. CONCLUSION： Chronic Ang Ⅱ infusion increases blood pressure of rats, and effects the morphology of islets β-cells, so its secretory function might be damaged.

关 键 词：血管紧张素Ⅱ 盐 胰岛Β细胞 高血压 大鼠 

分 类 号：R587.1[医药卫生—内分泌]