白细胞靶向超声造影显像无创评价犬心肌缺血再灌注损伤  

Noninvasive Imaging of Myocardial Ischemia-reperfusion Injury Using Leukocyte-targeted Contrast Echocardiography

作　　者：景香香[1] 王志刚[1] 李玲[1] 冉海涛[1] 李晓东[1] 彭晓琼[1] 杨春江[1] 

机构地区：[1]重庆医科大学超声影像学研究所附属第二医院超声科,重庆市400010

出　　处：《中国超声医学杂志》2006年第6期401-403,共3页Chinese Journal of Ultrasound in Medicine

基　　金：国家自然科学基金重点(No.30430230);面上项目(No.30370402);重庆市自然科学基金重点(CSTC;2005BA5024)项目

摘　　要：目的用白细胞靶向超声造影剂无创评价犬心肌缺血再灌注(ischemia-reperfusion,I-R)损伤的范围及严重程度。方法将自制“表活显”(self-made surfactant fluorocarbon-filledmicrobubbles,SFCMB)与磷脂酰丝氨酸(phosphatidylserine,PS)结合,制备成白细胞靶向超声造影剂(SFCMB-PS),在实时心脏超声造影(myocardialcontrastechocardiography,MCE)条件下,用SFCMB-PS对9只犬心肌I-R模型进行延迟心肌显像。实验结束后,在心肌I-R损伤处取组织块立即进行髓过氧化物酶(myeloperoxidase,MPO)活性测定。结果延迟心肌显像可见缺血心肌部位有明显充盈缺损,而再灌注后心肌的造影剂回声明显增强。定量分析结果表明I-R损伤心肌部位声像图的灰阶强度与MPO活性有明显的相关关系(r=0.776,P<0.05)。结论用白细胞靶向超声造影剂评价心肌I-R损伤有明显的临床价值。Objective To assess the region and severity of myocardium ischemia-reperfusion （I-R） injury via noninvasive ultrasound imaging using leukocyte-targeted contrast echocardiography. Methods We incorporated the phosphatidylserine （PS） into the shell of the self-made, surfactant fluorocarbon-filled microbubbles （SFCMB） for leukocyte-targeted ultrasound contrast agent （SFCMB-PS） . Nine open-chest canine myocardial ischemia-reperfusion models were established. Delayed myocardial imaging was applied by real-time myocardium contrast echocardiography （MCE） using SFCMB-PS. After experiment the myocardial tissues were cut in the region of I-R to determine tissue myeloperoxidase （MPO） activity. Results Delayed MCE imaging indicated that the clear filling defect was shown in the ischemic myocardium and the acoustic density after reperfusion was significantly increased. Quantitive analytical results demonstrated that it had an obvious correlation （r= 0. 776, P〈0.05 ）between the gray scale in the myocardial I-R region and MPO activity. Conclusions It has great clinical value for evaluating the myocardial I-R injury using leukocyte-targeted ultrasound contrast agent.

关 键 词：靶向性微泡 缺血再灌注 心脏超声造影 髓过氧化物酶 

分 类 号：R445.1[医药卫生—影像医学与核医学]