小鼠钟基因Period2(Per2)抑制乳腺癌细胞生长的研究  

Study on Inhibitory Effects of Mouse Circadian Gene Period2(Per2) on EMT6 Cells

作　　者：华慧[1] 王跃锜[1] 万朝敏[1] 刘延友[1] 王晓佳[1] 王正荣[1] 

机构地区：[1]四川大学华西医学中心基础医学与法医学院,四川成都610041

出　　处：《航天医学与医学工程》2006年第3期171-175,共5页Space Medicine & Medical Engineering

基　　金：国家自然科学基金资助(30470623;30470684)

摘　　要：目的研究小鼠节律基因Period2(Per2)对小鼠乳腺癌细胞(EMT6)的生长抑制及诱导凋亡的作用。方法将pcDNA3.1(+)-Per2转导入EMT6细胞内,同时设立转染空质粒pcDNA3.1(+)入细胞的对照组。以RT-PCR、Westernblot、流式细胞技术来检测Per2基因和蛋白的表达;采用MTT、细胞生长曲线、细胞平板集落形成实验检测Per2过表达后对小鼠乳腺癌细胞的生长抑制作用;通过流式细胞技术、DNA梯形带、hochst33258荧光染色和电镜技术检测Per2过表达后对小鼠乳腺癌细胞凋亡的影响。结果小鼠节律基因Per2过表达抑制EMT6细胞的生长和增殖,诱导细胞凋亡率增加。结论本研究发现小鼠节律基因Per2可能通过使EMT6细胞阻滞于G1期,并诱导细胞凋亡来实现对该肿瘤细胞的生长抑制作用。Objective To investigate the effects of mouse circadian gene Period2 （Per2） overexpression on growth inhibition and apoptosis of EMT6 cells. Method pcDNA3.1 （ ＋ ）-Per2 was transfected into EMT6 cells and paralleled with the vector control pcDNA3.1 （ ＋ ）. Per2 expression was confirmed by RT-PCR, western blot and flow cytometry. Inhibitory effect of Per2 on cell growth and proliferation were measured by MTT assay, cell count and colony-forming assay. Apoptosis induction of Per2 was detected by flowcytometry, DNA ladder electrophoresis and Hoechst33258 staining and electron microscope. Result The mouse circadian gene Per2 exhibited a statistically significant growth-inhibitory effect and apoptosis-inductory effect on EMT6 cells. Conclusion The results suggest Per2 may inhibit EMT6 cell proliferation by arresting the cells in G1 phase and inducing cellular apoptosis.

关 键 词：时间生物学 生物节律 节律基因 细胞周期 EMT6 

分 类 号：R852.6[医药卫生—航空、航天与航海医学]