将异体阔筋膜细胞外基质用于肾修补的实验研究  

Extracellular matrix of xenogenic femoral fascia for the repair of renal trauma

作　　者：黄广林[1] 王晓峰[1] 刘流[2] 李贵忠[2] 王建伟[2] 满立波[2] 胡杰[3] 

机构地区：[1]北京大学人民医院泌尿外科,北京100044 [2]北京积水潭医院泌尿外科 [3]清华大学生命科学院组织工程实验室

出　　处：《北京大学学报（医学版）》2006年第3期289-292,共4页Journal of Peking University:Health Sciences

基　　金：首都医学发展科研基金(20033055)资助~~

摘　　要：目的:探讨异体阔筋膜细胞外基质(extracellularmatrix,ECM)作为肾修补材料的效果。方法:12只犬随机分为术后第1,2周及术后第1,2,4,8个月6组,每组2只。术前及术后不同时段行血、尿常规,血肌酐、尿素氮及电解质检查,并做血清肾素测定。处死动物前分别测定左、右肾的肌酐清除率,处死动物后切取两侧肾分别称重并于修补局部取材行光镜及电镜检查。结果:补片覆盖缝合后创面均迅速止血,实验犬在术后均存活,无近远期并发症。术后各时段ECM补片与周围组织均无明显粘连,且补片大小形状无明显改变,无皱缩现象。术前及术后不同时段血、尿常规,肾功能、电解质及血清肾素均无明显变化。补片置入后各时期均无明显的炎性细胞浸润,随着时间的推移和宿主细胞的长入,补片逐渐变薄形成了接近正常的肾包膜结构。补片下方的肾皮质在术后各时期均无明显炎性细胞浸润。结论:异体阔筋膜细胞外基质为理想的肾修补材料。Objective： To study the effect of extracellula matrix of xenogenic femoral fascia in repair of renal trauma. Methods： Twelve adult dogs were used and randomly assigned to 6 groups, and the animals were sacrificed separately in 1 and 2 weeks 1,2,4 and 8 months after renal repair operations. The examinations of blood and urine routine, blood urea nitrogen and creatinine, electrolyte and serum renin were performed before and after operations at various times. The creatinine clearances of affected and contralateral normal kidneys were evaluated before death and the local areas of renal repair were studied by light and electron microscopy. Results： Bleeding was stopped completely after the entire patch was sutured, and only mild adhesions to around tissues were found in various times after operations. As time passed, the repair patch was replaced by smooth neocapsule like normal renal capsule. Conclusion： ExtraceUula matrix of xenogenic femoral fascia might be an ideal tissue-engineering material for renal repair.

关 键 词：阔筋膜 细胞外基质 肾 修复外科手术 

分 类 号：R699.2[医药卫生—泌尿科学]