大鼠骨髓间质干细胞对同种骨髓移植后造血重建和免疫重建的作用  被引量:10

Effects of cotransplantation of rat bone marrow mesenchymal stem cells and bone marrow on hematopoiesis

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作  者:雷俊霞[1] 郭振宇[2] 赵东长[3] 李红霞[4] 余伟华[1] 张秀明[5] 郑芹[1] 魏箐[6] 李树浓[1] 项鹏[5] 

机构地区:[1]中山大学中山医学院病理生理教研室 [2]中山大学附属第五医院器官移植科,广东珠海519000 [3]广东省妇幼保健院儿科,广东广州510010 [4]广州市公安局刑事科学技术研究所,广东广州510030 [5]中山大学干细胞中心,广东广州510080 [6]中山大学附属第二医院医学中心,广东广州510120

出  处:《中国病理生理杂志》2006年第6期1129-1132,共4页Chinese Journal of Pathophysiology

基  金:国家自然科学基金资助项目(No.30100188);广州市科技计划项目(No.2002U13E0011)资助

摘  要:目的:探讨大鼠骨髓间质干细胞(MSC)对同种异体骨髓移植造血重建和免疫重建的影响。方法:建立大鼠同种异体骨髓移植模型,通过生存率分析、外周血象检测、免疫细胞计数和受体免疫功能检测,综合评价MSC对骨髓移植(bone marrowtransplantation,BMT)后造血重建和免疫重建的作用。结果:(1)MSC可促进BMT后造血重建:移植后30d,共移植组外周血白细胞、淋巴细胞和血小板数均高于单纯骨髓移植组;共移植组骨髓细胞数也高于对照组。(2)MSC可促进BMT后免疫重建:移植后30d,共移植组胸腺细胞数、脾细胞总数均高于骨髓单纯移植组;共移植组对ConA、LPS刺激的淋巴细胞增殖反应以及对第三体来源的同种混合淋巴细胞反应均强于单纯BMT组。结论:大鼠MSC与骨髓共移植对同种异体骨髓移植造血重建和免疫重建有一定促进作用。AIM: To investigate effects of rBMMSC on hematopoiesis and immune reconstitution after allo-hematopoietic stem cells transplantation (HSCT).METHODS:Allogeneic BMT model from Fischer344 rats (RT - 1A1) to Wistar rats (RT-1Au) was established. The effects of MSCs on hematopoietic reconstitution and immune reconstitution were studied by observing the survival rate, peripheral blood counts, thymus counts, spleen counts, bone marrow counts and immune function analysis at 30 days after transplantation. RESULTS: 1. Cotransplantation of MSCs and bone marrow (BM) was demonstrated to improve hematopoiefic reconstitution. Lymphocyte and platelet counts in peripheral blood in cotransplantation groups were higher than those in control groups. More bone marrow neucleated cells were also observed in cotransplantation groups. 2. Cotransplantation of MSCs and BM improved immune reconstitution. First, overall thymic cellularity and spleen cellularity significantly increased in cotransplantation groups at day 30. Secondly, cotransplantation improved immune functional recovery. Non-specific lymphocytes proliferation reaction induced by ConA and LPS increased in cotransplantation group, and so did for allogeneic mixed-lymphocyte reaction. CONCLUSION: Hematopoietic reconstitufion and immune reconsfitution were significantly enhanced by MSCs cotransplanted with BM.

关 键 词:间质干细胞 骨髓移植 造血复建 免疫复建 

分 类 号:R338.8[医药卫生—人体生理学]

 

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