人类重组酸性成纤维细胞生长因子对细胞增殖和凋亡的影响  

Effects of human recombinant acidic fibroblast growth factor on cell proliferation and apoptosis in NIH3T3 cells

作　　者：吕文天[1] 赵红光[1] 龚平生[2] 王志成[1] 姜晓燕[1] 李校堃[3] 龚守良[1] 

机构地区：[1]吉林大学公共卫生学院卫生部放射生物学重点实验室,吉林长春130021 [2]暨南大学医药生物技术开发中心 [3]吉林大学生命科学院酶工程实验室,吉林长春130021

出　　处：《中国病理生理杂志》2006年第6期1142-1146,共5页Chinese Journal of Pathophysiology

基　　金：国家863专题项目(No.2002AA2Z3318)

摘　　要：目的:通过对人类重组型和野生型酸性成纤维细胞生长因子(raFGF,waFGF)的比较研究,探讨raFGF与促分裂性有关的生物学作用。方法:应用NIH3T3细胞株,用MTT法通过细胞增殖实验检测waFGF和raFGF的促分裂性,通过流式细胞术检测细胞凋亡、细胞膜通透性和线粒体膜电位。同时检测肝素(HS)对aFGF生物学作用的影响。结果:raFGF对细胞增殖作用明显低于waFGF。raFGF对细胞凋亡的抑制作用及其对细胞膜通透性的增强作用也明显低于waFGF,对线粒体膜电位的增强作用明显低于waFGF。HS对aFGF的生物学作用具有促进作用。结论:raFGF的促分裂作用低于waFGF,而对细胞凋亡的影响不明显。AIM： Through studying the differences between wild - type acidic fibroblast growth factor （waFGF） with recombinant aFGF （raFGF）, the biological effect of raFGF concerned with mitogenic activity was evaluated. METHODS： NIH3T3 cell line was used. Cell proliferation with MTr method was used to study the mitogenic activity. Flow cytometry was also used for detection of apoptosis, cell membrane permeability and mitochondria potential. The role of heparin sulfate （HS） on aFGF biological effect was studied at the same time in this research. RESULTS： The enhancement of raFGF on cell proliferation was significantly lower than that of waFGF. The restriction of raFGF on apoptosis and the enhancement of it on cell membrane permeability were all lower than those of waFGF significantly. The enhancement of raFGF on mitochondria potential was lower than that of waiF significantly. The HS improved the biological effect of aFGF. CONCLUSION： The mitogenic activity of raFGF is lower than that of waFGF and raFGF has little effect on apoptosis.

关 键 词：成纤维细胞生长因子1 肝素 细胞膜通透性 线粒体 膜电位 细胞凋亡 

分 类 号：R363[医药卫生—病理学]