参与小鼠神经发育的mCcd1基因的组织表达  

Tissue Expression of mCcd1-a,a Gene Involved in Mouse Neural Development

作　　者：马鑫[1] 景孝堂[1] 刘淑红[1] 吴燕[1] 范文红[1] 王子仁[2] 范明[1] 

机构地区：[1]军事医学科学院基础医学所,北京100850 [2]兰州大学生命科学学院,兰州730000

出　　处：《动物学杂志》2006年第3期27-30,共4页Chinese Journal of Zoology

基　　金：国家863基金资助(No.2002BA711A01-03)

摘　　要：介绍了一种可能在神经发育过程中起重要调节作用的基因mCcd1在小鼠发育过程中的mRNA和蛋白质水平的表达变化。通过对新生和成年小鼠多组织免疫杂交(Western Blot)和反转录PCR(RT-PCR)检测发现:该基因在多种组织中广泛表达,但脑部显示高表达,并且新生鼠各组织表达均高于成体。小鼠11.5 d胚胎切片免疫组化实验也支持这一结果。mCcd1在神经系统发育早期的表达暗示它可能参与了神经系统的发育过程。Ccdl （coiled-coil-DIX1） is a novel gene that may regulate the development of nervous system. We studied the developmental expression pattern of mCcdl at protein and mRNA levels in mice. The results of Western blot and RT-PCR on both postnatal and adult mice indicate that the expression of Ccdl is mainly concentrated in the brain, and its expression in tissues of the postnatal mice is higher than that in the adult. Furthermore, immunohistochemistry of prenatal 11.5 d embryos also supports this conclusion. Altogether, Ccdl may play an important role in the development of nervous system as well as other systems in mouse.

关 键 词：mCcd1基因 神经发育 基因表达 小鼠 

分 类 号：Q954.48[生物学—动物学] Q953